FastQCFastQC Report
Thu 5 Dec 2024
367_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename367_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19352690
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7616003.935370225017814Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3201941.654519345889383Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG2351941.2153039189900732No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGGGGG1741310.8997767235459256TruSeq Adapter, Index 16 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGGGGG1590220.8217048896044943TruSeq Adapter, Index 16 (97% over 43bp)
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC1254660.6483129735452797No Hit
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA897980.4640078459376965Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC726890.3756015313633402Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG560970.28986668003259497No Hit
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC535970.2769485792414388No Hit
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC490380.2533911306386864No Hit
TGATCTGAACTTTTTATCACATTTAAGCCAGATCGGAAGAGCACACGTCT484910.25056465018558144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC480850.2484667506170977No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGGAGATCGGAAGAGCACACGTCT399500.2064312506426755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC387530.20024606398386996No Hit
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC372370.19241252766411285Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGGGTG362210.18716261150258698TruSeq Adapter, Index 16 (97% over 43bp)
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC362080.18709543737847298Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT357810.18488902576334348No Hit
TGTAGTTCTTCCAGATGTCTCAGATATTGAGATCGGAAGAGCACACGTCT353270.18254309865966953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTTGGTCTGCAAAGTTGTCTTTATAGCAGATCGGAAGAGCACACGTCT310350.16036530322141263Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATCGTTAAGACAATGATGTCCAAAGCAGATCGGAAGAGCACACGTC309130.1597348999028042No Hit
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG263160.13598109616802626No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTTAGATCGGAAGAGCACACGTC258750.1337023431884663No Hit
TAAGATCTCTAGATTATCCATTTTGTCTGAGATCGGAAGAGCACACGTCT251080.12973906986573958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTGAACTTTTTATTACATTGCAACCCAGATCGGAAGAGCACACGTC230680.11919789962015616No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGTGGG217650.11246498548780556TruSeq Adapter, Index 16 (97% over 43bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT206170.10653299360450666No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTGAGATCGGAAGAGCACACGTC200580.10364450626760414No Hit
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC196750.10166545322639901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)