FastQCFastQC Report
Thu 5 Dec 2024
363_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename363_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20926097
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA8057093.850259319738411Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA5125402.4492861712339384Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG3364811.6079491555448682Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT2400371.1470700914747742No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC2136911.0211698817987893No Hit
GGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC1175320.5616527534972241Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1097910.5246606665351882Illumina RNA PCR Primer (100% over 50bp)
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT1081850.5169860390114793No Hit
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA905980.43294265528827475Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT784250.3747712724451196No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT774500.3701120185001532No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG613520.29318415182726143Illumina Small RNA Adapter 1 (100% over 26bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG516390.2467684250914062No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT475960.22744805206627877Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC454030.21696831473160044Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC376240.17979463633376067Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG374790.17910172164450924Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTCTGGCAGACGTCACAAAGATGTAAAAGATCGTCGGACTGTAGAACTC357520.17084886875942512Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACGGAATTATCTATTTCCCAGAAGATCACAGATCGTCGGACTGTAGAACT324260.1549548394045961No Hit
CCAGGTGTAAGCACCAAAAGACCGATACAGATCGTCGGACTGTAGAACTC314750.15041027478750577Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT293460.14023637566049704No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC284080.13575393442933958No Hit
ACCAGGTGTAAGCACCAAAAGACCGATACAGATCGTCGGACTGTAGAACT279310.13347448403780218No Hit
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC253950.12135564505889464Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTTCAGTGAGATTTGTCAACATGAAAATTCTGACCCGAAAGATGGACATC239130.11427357906254568No Hit
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC239040.11423057056459214No Hit
CACGAGCCGAGTGATCCACCGCATAGAGTTGGATCGTCGGACTGTAGAAC230090.10995361437921272No Hit
AAAATGAAACCTCACAACATCCTAGACAAAGATCGTCGGACTGTAGAACT213940.10223597835755038No Hit
GATTCACAAAAGAACACAAATCTTGATTCAGATCGTCGGACTGTAGAACT211590.10111297868876362No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)