FastQCFastQC Report
Thu 5 Dec 2024
363_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename363_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20926097
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7475063.572123363472892Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGG3974361.8992361547401793TruSeq Adapter, Index 15 (97% over 43bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3104271.4834443326913758Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC2361891.1286815692386403No Hit
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG2073370.9908058822435928No Hit
TGATCTGAACTTTTTATCACATTTAAGCCAGATCGGAAGAGCACACGTCT1149910.5495100209083423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC1003840.4797072287297531No Hit
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA895170.4277768568118555Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG758870.3626428760222224No Hit
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC740810.3540125040995461No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTAGGGGG682930.32635326119342756TruSeq Adapter, Index 15 (97% over 43bp)
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC605530.2893659529533864Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC520960.24895230104304686No Hit
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC506090.2418463414367237No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC444460.21239507778254113Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGGGGG426810.20396063346165316TruSeq Adapter, Index 15 (97% over 39bp)
TGATCTGAACTTTTTATCACATTTAAGCTAGATCGGAAGAGCACACGTCT414410.19803501818805486Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC369260.1764590883813642Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATCTGAACTTTTTATCACATTGCAACCAGATCGGAAGAGCACACGTCT367790.1757566162481231Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTACATCTTTGTGACGTCTGCCAGAGCAGATCGGAAGAGCACACGTCT342800.16381458998302453Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGATCTTCTGGGAAATAGATAATTCCGTAGATCGGAAGAGCACACGTC307410.14690269284329513No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGGAGATCGGAAGAGCACACGTCT289910.13853992935232978Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT276540.13215077804523223No Hit
TGATCTGAACTTTTTATCACATTGCAACCAAGATCGGAAGAGCACACGTC267670.1279120516358115No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGGTAGATCGGAAGAGCACACGTC259240.1238835889941636No Hit
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC247480.11826381192823487No Hit
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG240360.11486136186791068No Hit
TAAGATCTCTAGATTATCCATTTTGTCTGAGATCGGAAGAGCACACGTCT229460.10965255489353797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT215960.10320128020050753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)