FastQCFastQC Report
Thu 5 Dec 2024
35_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename35_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17663366
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA6627023.751844354014971Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3583202.0286054198276817Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG3160231.7891437000173125Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC1903321.0775522626887764No Hit
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT1482540.8393303971621264No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG843670.4776382938563352Illumina Small RNA Adapter 1 (100% over 26bp)
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA748680.4238603219794007Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT717840.40640045617579346Illumina RNA PCR Primer (100% over 50bp)
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT635490.3597785382469004No Hit
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT503870.28526272965186816No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT497830.28184322286024077No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT489490.27712158599895403Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG458570.2596164287146629No Hit
GGGTTGCAATGTAATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT429600.24321525127203955No Hit
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG356180.20164899487447635Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTTCAATTACTTCAAAGAAAACTAGACCAGATCGTCGGACTGTAGAACT335310.1898335798510884No Hit
AATCTTCATAAAGAACACAAGAGACATAGAGATCGTCGGACTGTAGAACT325180.18409854610950144No Hit
AAGATGTTAAGGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT303910.17205667368269445No Hit
CAGTCTTGCCAGAGAATTGAATGTCCTGCAGATCGTCGGACTGTAGAACT286740.16233598964093254No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC278370.15759736847438932No Hit
GTGCTCTTGTAATGAGGAGCTTCAGACCTTAGATCGTCGGACTGTAGAAC277470.15708783931669648No Hit
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC237900.13468554068346883Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTTCAGTGAGATTTGTCAACATGAAAATTCTGACCCGAAAGATGGACATC230030.13022999127119939No Hit
ACCTGTTGGGAAGTCTACAGATCTAGTTCAGATCGTCGGACTGTAGAACT219460.12424585438585148No Hit
GCTGAATTTATAAACAGATACGAAGCTGGAGATCGTCGGACTGTAGAACT214710.1215566727202505No Hit
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC209810.11878256952836735No Hit
AAAATGAAACCTCACAACATCCTAGACAAAGATCGTCGGACTGTAGAACT202290.11452517034408957No Hit
TGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT199150.11274747972725017No Hit
ACGGAATTATCTATTTCCCAGAAGATCACAGATCGTCGGACTGTAGAACT198230.1122266276993864No Hit
AAATTGTTGAAGCTTTAGACCGAGTATTCAGATCGTCGGACTGTAGAACT197680.11191524876968523No Hit
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG197600.11186995728900143Illumina Small RNA Adapter 1 (100% over 26bp)
AATATAATTGATTCTACACAACAATATACTTAAGATCGTCGGACTGTAGA193130.10933929580579375No Hit
GGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC185730.10514983384254167Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGTTGCAATGTAATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT180720.1023134548647183No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)