FastQCFastQC Report
Thu 5 Dec 2024
35_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename35_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17663366
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6341063.5899499563107056Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3011751.7050827118681684Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG1858251.052036174758537No Hit
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC1476390.8358486145845587No Hit
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC854120.48355449352065744Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGGGGG754380.4270873399781219TruSeq Adapter, Index 6 (97% over 45bp)
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA746220.42246760894837376Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTGTG737060.4172817344100779TruSeq Adapter, Index 6 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTGGG606380.3432981007130804TruSeq Adapter, Index 6 (97% over 46bp)
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC601490.34052965895628273No Hit
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG488780.2767196241078852No Hit
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC487080.2757571801433543No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC467460.26464944450565087Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC451570.25565342415482983No Hit
TGATCTGAACTTTTTATTACATTGCAACCCAGATCGGAAGAGCACACGTC428240.24244529610041482No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTATG371520.2103336362955962TruSeq Adapter, Index 6 (97% over 49bp)
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC355120.20104888275541594Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGTCTAGTTTTCTTTGAAGTAATTGAAGTAGATCGGAAGAGCACACGTC320360.18136973439830212No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATTAGATCGGAAGAGCACACGTC310760.17593475671624537No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC294440.1666952946567489No Hit
TATCGAACTATTGATGAACCTTAACATCTTAGATCGGAAGAGCACACGTC293680.16626502559025275No Hit
TAAGGTCTGAAGCTCCTCATTACAAGAGCACAGATCGGAAGAGCACACGT278660.1577615500918681No Hit
TGCAGGACATTCAATTCTCTGGCAAGACTGAGATCGGAAGAGCACACGTC274090.1551742742578057No Hit
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT271710.15382685270746244No Hit
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC229170.12974310785384846No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGGGCGCAGC229020.12965818632756634No Hit
TAAGATCTCTAGATTATCCATTTTGTCTGAGATCGGAAGAGCACACGTCT226590.1282824576017957Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAACTAGATCTGTAGACTTCCCAACAGGTAGATCGGAAGAGCACACGTC211120.11952421752456467No Hit
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG210750.11931474442640208No Hit
TCCAGCTTCGTATCTGTTTATAAATTCAGCAGATCGGAAGAGCACACGTC209680.11870897087225617No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTGGGGGG196190.11107169494194934TruSeq Adapter, Index 6 (97% over 45bp)
TTTGTCTAGGATGTTGTGAGGTTTCATTTTAGATCGGAAGAGCACACGTC192820.10916379131814401No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTTAGATCGGAAGAGCACACGTC192150.10878447516741711No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGTAGATCGGAAGAGCACACGTC191430.10837685184126288No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGGGTG186600.10564237869497807TruSeq Adapter, Index 6 (97% over 45bp)
TATCACAGCTGGCTTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTC186260.10544988990207188Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATCTGAACTTTTTATCACATTTAAGCCAGATCGGAAGAGCACACGTCT184910.10468559616553265Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTGAACTTTTTATCACATTGCAACCAAGATCGGAAGAGCACACGTC184020.10418172844292531No Hit
TTAAGTATATTGTTGTGTAGAATCAATTATATTAGATCGGAAGAGCACAC178100.10083015887232365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)