FastQCFastQC Report
Thu 5 Dec 2024
34_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename34_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22627992
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA7501223.315017965359012Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4103451.8134397431287763Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG3447221.523431685851754Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC1682350.743481790165031No Hit
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT1548700.6844177777683499No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG965650.4267501950681263Illumina Small RNA Adapter 1 (100% over 26bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT891980.39419317454239866Illumina RNA PCR Primer (100% over 50bp)
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA878100.38805917909110094Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT606600.26807504616406086No Hit
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT602170.2661172940135387No Hit
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT586230.2590729217157227No Hit
GGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC547040.24175366510647522Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG476170.2104340500031996No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT406960.17984803954323478Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG401550.17745719549485434Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCTTCATAAAGAACACAAGAGACATAGAGATCGTCGGACTGTAGAACT316870.14003452007584236No Hit
CTTTGGTTAGCCCTCAGAAATCAACAGTTGGATCGTCGGACTGTAGAACT293840.1298568604761748No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC276960.12239707350082146No Hit
GGGTTGCAATGTAATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT270870.1197057167069884No Hit
GTTCAGTGAGATTTGTCAACATGAAAATTCTGACCCGAAAGATGGACATC257000.11357614056077092No Hit
CATTCCATCCAGATGGCGAACCGAGACAAAGATCGTCGGACTGTAGAACT250000.11048262700464098No Hit
ACGGAATTATCTATTTCCCAGAAGATCACAGATCGTCGGACTGTAGAACT247080.10919218992122676No Hit
AATATAATTGATTCTACACAACAATATACTTAAGATCGTCGGACTGTAGA245530.108507197633798No Hit
GCTTTGGACATCATTGTCTTAACGATCGGAGATCGTCGGACTGTAGAACT243520.1076189173126807No Hit
CCAGGTGTAAGCACCAAAAGACCGATACAGATCGTCGGACTGTAGAACTC238240.10528552423034265Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)