FastQCFastQC Report
Thu 5 Dec 2024
34_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename34_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22627992
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7246933.202639456474971Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGGGGG3998141.766900041329341TruSeq Adapter, Index 5 (97% over 45bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3309511.4625734355925175Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG1646510.7276430007576457No Hit
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC1571090.6943126018428856No Hit
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC986320.43588489866887Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA881820.38970316058093Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG595030.26296191018628606No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGGGGGGGGGG575760.25444590929676836TruSeq Adapter, Index 5 (97% over 41bp)
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC575610.25437961972056555No Hit
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC572690.25308918263715136No Hit
TGATCTGAACTTTTTATCACATTTAAGCCAGATCGGAAGAGCACACGTCT552100.24398983347704914Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC467720.2066997372104427No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC421020.18606158248597576No Hit
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC401100.17725832676624598Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC392860.17361681938017304Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTATGTCTCTTGTGTTCTTTATGAAGATTAGATCGGAAGAGCACACGTC304770.13468716092881772No Hit
CAACTGTTGATTTCTGAGGGCTAACCAAAGAGATCGGAAGAGCACACGTC286930.12680312066576654No Hit
TGATCTGAACTTTTTATTACATTGCAACCCAGATCGGAAGAGCACACGTC273990.12108453989200633No Hit
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT269830.11924610897864911No Hit
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC259350.11461467725461455No Hit
TTTGTCTCGGTTCGCCATCTGGATGGAATGAGATCGGAAGAGCACACGTC243660.10768078758380328No Hit
TCCGATCGTTAAGACAATGATGTCCAAAGCAGATCGGAAGAGCACACGTC243050.10741120997391196No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGTAGATCGGAAGAGCACACGTC242130.10700463390653488No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGGAGATCGGAAGAGCACACGTCT228610.1010297334381239Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAGTATATTGTTGTGTAGAATCAATTATATTAGATCGGAAGAGCACAC228520.10098995969240222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)