FastQCFastQC Report
Thu 5 Dec 2024
341_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename341_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19715666
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA10567475.359935596393243Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4867002.468595278495791Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG4406802.2351768385607667Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC2142681.0867905755757883No Hit
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1125600.5709165493065261Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT996530.5054508429996735Illumina RNA PCR Primer (100% over 50bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT952110.4829205363896913No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG776550.3938745969829272Illumina Small RNA Adapter 1 (100% over 26bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG754330.3826043715692891No Hit
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT642690.32597935063416067No Hit
ACGGAATTATCTATTTCCCAGAAGATCACAGATCGTCGGACTGTAGAACT524470.26601688220930503No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT471090.23894196625160927Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTTGTTCTTTTATGGTACGAACGTTGATCAGATCGTCGGACTGTAGAAC471080.23893689414296224No Hit
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG467440.23709064659545356Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCTTCATAAAGAACACAAGAGACATAGAGATCGTCGGACTGTAGAACT442030.22420241852342196No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC328740.1667404996615382No Hit
ACCAGGTGTAAGCACCAAAAGACCGATACAGATCGTCGGACTGTAGAACT317820.1612017570190122No Hit
AACCTCAGCTTCTACACTGGAAAGACTAGAGATCGTCGGACTGTAGAACT317420.16099887267313212No Hit
CCAGGTGTAAGCACCAAAAGACCGATACAGATCGTCGGACTGTAGAACTC310640.15755998301046487Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGTCTTGAAGTCCTGAGCGATTTCACGTAGATCGTCGGACTGTAGAACT249000.12629550531034558No Hit
GGACAGACCAGATTCACAAGCACAGACTTAGATCGTCGGACTGTAGAACT233560.11846416955937478No Hit
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC229970.1166432825551011No Hit
GTACAGACCAGATCCAAAAGCACAGACTTAGATCGTCGGACTGTAGAACT213610.10834531280860611No Hit
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG208660.10583461902834021Illumina Small RNA Adapter 1 (100% over 26bp)
ACTTGTTCAAAGTTAGATATACTGAAGATGAGATCGTCGGACTGTAGAAC208060.10553029250952009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)