FastQCFastQC Report
Thu 5 Dec 2024
341_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename341_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19715666
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9752004.946320352556185Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC4045492.051916481035944Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGGGG2461041.2482662264617386TruSeq Adapter, Index 14 (97% over 43bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG2074641.052279948341588No Hit
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1110680.5633489632051993Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGGGG1087220.5514497963193331TruSeq Adapter, Index 14 (97% over 43bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG930090.4717517531489933No Hit
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC761770.3863780204026585Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC738920.37478825214425926No Hit
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC604480.30659882349396667No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGTAGATCGGAAGAGCACACGTC494690.25091214265853357No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC468390.23757249691691876No Hit
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC458130.23236851344509488Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATCAACGTTCGTACCATAAAAGAACAAGTAGATCGGAAGAGCACACGT444780.22559724840134746No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC434870.22057078873216862Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTATGTCTCTTGTGTTCTTTATGAAGATTAGATCGGAAGAGCACACGTC409470.20768763276878396No Hit
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT320130.1623734141164696No Hit
TCTAGTCTTTCCAGTGTAGAAGCTGAGGTTAGATCGGAAGAGCACACGTC297820.15105753972500854No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGGTAGATCGGAAGAGCACACGTC294760.14950547447902596No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGGTG290250.14721795347922814TruSeq Adapter, Index 14 (97% over 43bp)
TGTATCGGTCTTTTGGTGCTTACACCTGGAGATCGGAAGAGCACACGTCT285080.14459567330872822Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTGGG233880.11862647703607881TruSeq Adapter, Index 14 (97% over 43bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG231890.11761712741532546No Hit
TAAGTCTGTGCTTGTGAATCTGGTCTGTCCAGATCGGAAGAGCACACGTC231090.1172113587235653No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTGAGATCGGAAGAGCACACGTC230760.11704397913821424No Hit
TAAGTCTGTGCTTTTGGATCTGGTCTGTACAGATCGGAAGAGCACACGTC211130.10708742986414967No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)