FastQCFastQC Report
Thu 5 Dec 2024
33_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19918975
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA5855832.9398249658930746Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3793871.904651218247927Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG2667201.3390247239127515Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT2318471.1639504542778933No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC1731460.869251555363667No Hit
GCTATAAAGACAACTTTGCAGACCAAATAGATCGTCGGACTGTAGAACTC1621250.8139224031357035Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT1058530.5314179067949029No Hit
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT891960.44779412595276613No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT845400.4244194292125975Illumina RNA PCR Primer (100% over 50bp)
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG784200.39369495669330373Illumina Small RNA Adapter 1 (100% over 26bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT686740.34476673624019305No Hit
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA662440.33256731332812056Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC578110.2902307975184466Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATCTTCATAAAGAACACAAGAGACATAGAGATCGTCGGACTGTAGAACT498510.25026890188877693No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG436100.21893696839320295No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT421390.21155205024354917Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAATATCTGAGACATCTGGAAGAACTACAGATCGTCGGACTGTAGAACTC400900.2012653763559621Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTTGTTCTTTTATGGTACGAACGTTGATCAGATCGTCGGACTGTAGAAC361340.18140491666865388No Hit
ACTATAAAGACAACTTTGCAGACCAAATAGATCGTCGGACTGTAGAACTC318930.1601136604669668Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG302460.15184516271545095Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCCAAAATACCCGACGGTTCACATATAGATCGTCGGACTGTAGAACTC297140.14917434255527706Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT278430.1397812889468459No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC259020.13003681163312872No Hit
CATGTTTTTATGAAACAAACACAGACCTTAGATCGTCGGACTGTAGAACT252010.12651755424162137No Hit
GGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC237740.1193535309924331Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATGAACAGATAATTTCTATTGGATCCAGATCGTCGGACTGTAGAACTCT217700.10929277234395846Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCAGTGAGATTTGTCAACATGAAAATTCTGACCCGAAAGATGGACATC214330.10760091821993853No Hit
AACCTCAGCTTCTACACTGGAAAGACTAGAGATCGTCGGACTGTAGAACT213670.10726957586924026No Hit
GAAGGCCATTACACGCCACCAGACCAGTGAGATCGTCGGACTGTAGAACT212430.10664705387701927No Hit
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC206690.10376537949367375Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)