FastQCFastQC Report
Thu 5 Dec 2024
33_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19918975
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5601552.812167794778597Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGG3055161.5337937820595688TruSeq Adapter, Index 4 (97% over 45bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC2533991.2721487927968183Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC2348841.1791972227486605No Hit
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG1683130.8449882586829894No Hit
TATTTGGTCTGCAAAGTTGTCTTTATAGCAGATCGGAAGAGCACACGTCT1601620.8040674783717536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC1030420.5173057348583449No Hit
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC836870.4201370803467548No Hit
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC807830.40555801691603105Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG669640.3361819571539198No Hit
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA661970.3323313574117142Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTAGGGGG641050.32182880896230853TruSeq Adapter, Index 4 (97% over 45bp)
TGATCTGAACTTTTTATCACATTTAAGCCAGATCGGAAGAGCACACGTCT584230.293303244770376Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGGGGGGG551850.27704738823157316TruSeq Adapter, Index 4 (97% over 41bp)
TCTATGTCTCTTGTGTTCTTTATGAAGATTAGATCGGAAGAGCACACGTC477730.23983663818042847No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC447430.2246250120801899No Hit
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC425750.21374091789361652No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC405130.20338897960361915Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAGTTCTTCCAGATGTCTCAGATATTGAGATCGGAAGAGCACACGTCT385220.1933934853575548Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCAACGTTCGTACCATAAAAGAACAAGTAGATCGGAAGAGCACACGT352800.1771175474641642No Hit
TATATGTGAACCGTCGGGTATTTTGGACCAGATCGGAAGAGCACACGTCT300070.15064530177883148Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC299920.15056999669912735Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATTTGGTCTGCAAAGTTGTCTTTATAGTAGATCGGAAGAGCACACGTCT285190.14317503787217967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTGAACTTTTTATCACATTGCAACCAAGATCGGAAGAGCACACGTC259720.13038823533841476No Hit
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT250980.1260004593609862No Hit
TAAGGTCTGTGTTTGTTTCATAAAAACATGAGATCGGAAGAGCACACGTC243490.12224022571442555No Hit
TGATCTGAACTTTTTATCACATTGCAACCAGATCGGAAGAGCACACGTCT241170.1210755071483347Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC217940.1094132604714851No Hit
TGGATCCAATAGAAATTATCTGTTCATTAGATCGGAAGAGCACACGTCTG210260.10555764039063253Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACTGGTCTGGTGGCGTGTAATGGCCTTCAGATCGGAAGAGCACACGTC208720.10478450823900326No Hit
TCTAGTCTTTCCAGTGTAGAAGCTGAGGTTAGATCGGAAGAGCACACGTC207100.10397121337819844No Hit
TACGAATATCCGGATCTGAAAACTGAATTCAGATCGGAAGAGCACACGTC200090.10045195598669109No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)