FastQCFastQC Report
Thu 5 Dec 2024
310_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename310_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19737649
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7863723.9841219184716476Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGGG3486211.7662741900010481TruSeq Adapter, Index 13 (97% over 43bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3370001.7073968637298191Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG3054561.547580464117079No Hit
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC2081911.0547912773198065No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTAGGGGG1174210.5949087452107391TruSeq Adapter, Index 13 (97% over 43bp)
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC974210.4935795544849338Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA883370.44755583605727306Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC843050.4271278712069507No Hit
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG714720.3621099959777378No Hit
TGATCTGAACTTTTTATCACATTTAAGCCAGATCGGAAGAGCACACGTCT624000.3161470750645125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC579230.293464535720541Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGGGGGGGG548650.27797130245856533TruSeq Adapter, Index 13 (97% over 39bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC543750.2754887372857831No Hit
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC478160.2422578291872553No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATTAGATCGGAAGAGCACACGTC463750.23495706099546104No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC453220.22962207910374738No Hit
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC372440.18869521896959462Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT341910.17322731800530042No Hit
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG334060.16925014726931256No Hit
TGATCTGAACTTTTTATTACATTGCAACCCAGATCGGAAGAGCACACGTC291370.14762143150888946No Hit
TGTCTGTCGGCATTTGACGGCATCTGCCACAGATCGGAAGAGCACACGTC266730.13513767521147022No Hit
TGATCTGAACTTTTTATCACATTGCAACCAGATCGGAAGAGCACACGTCT249860.12659055797374855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCACAGCTGGCTTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTC239840.12151396551838571Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTCCATGTTCTCTCTGATATTTGAAGTTAGATCGGAAGAGCACACGTC222980.11297191474020031No Hit
TGTGTCTTTATCGCTTTGTACAATAGTAGTAGATCGGAAGAGCACACGTC220970.11195355637340597No Hit
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC218800.11085413465403099No Hit
TGATCTGAACTTTTTATCACATTTAAGCTAGATCGGAAGAGCACACGTCT214960.10890861419209552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC209790.10628925461183346Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGCTATATCATCAGGATCTGTGAATATAGATCGGAAGAGCACACGTCTG208510.10564074779118832Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGATCTGAACTTTTTATCACATTGCAACCAAGATCGGAAGAGCACACGTC207270.10501250680868832No Hit
TCCGATCGTTAAGACAATGATGTCCAAAGCAGATCGGAAGAGCACACGTC201590.10213475779207544No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)