FastQCFastQC Report
Thu 5 Dec 2024
30_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename30_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19810124
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA5473502.762981190829497Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4710762.3779558371265117Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG2699841.3628587080020298Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT2367511.1951010503518302No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC2228281.124818804768713No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT1188030.5997085126776592No Hit
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT1098320.5544235866469084No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG1007280.5084672867267261Illumina Small RNA Adapter 1 (100% over 26bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT986590.498023132010683Illumina RNA PCR Primer (100% over 50bp)
GGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC799010.40333417398094024Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG774470.39094656853233223No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT701460.35409167554933024Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA614800.3103463663326893Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTGGCAGATGCCGTCAAATGCCGACAGACAGATCGTCGGACTGTAGAACT332900.16804538931709867No Hit
AGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC317260.16015043621130287Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT310900.1569399565595854No Hit
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG297850.15035241576478775Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTCTTGCCAGAGAATTGAATGTCCTGCAGATCGTCGGACTGTAGAACT277410.14003445914826176No Hit
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG277260.13995874028855146Illumina Small RNA Adapter 1 (100% over 26bp)
GGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC273660.13814148765550383Illumina DpnII expression Sequencing Primer (100% over 21bp)
CACGAGCCGAGTGATCCACCGCATAGAGTTGGATCGTCGGACTGTAGAAC267740.13515311665893662No Hit
GCTTTTACAACCGAAAGAAACACAACGTAGATCGTCGGACTGTAGAACTC267130.13484519329611466Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATGAAGAGGTTCTCTCTATAGCCCGGACATAGATCGTCGGACTGTAGAAC260970.13173567212401094No Hit
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC250400.12640001647642388No Hit
GAAGAGGTTCTCTCTATAGCCCGGACATAGATCGTCGGACTGTAGAACTC250210.1263041059207908Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATGAACAGATAATTTCTATTGGATCCAGATCGTCGGACTGTAGAACTCT237510.11989324246531723Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCTCTACAAGATAGTAAGACAGTTCTACAAGATCGTCGGACTGTAGAACT225710.11393669216810556No Hit
AAATTGTTGAAGCTTTAGACCGAGTATTCAGATCGTCGGACTGTAGAACT221210.11166512637679604No Hit
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT220360.11123605283843754No Hit
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC209410.10570857607958435Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA205890.10393170683838224Illumina RNA PCR Primer (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)