FastQCFastQC Report
Thu 5 Dec 2024
30_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename30_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19810124
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5246662.648474083251574Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGGG2643801.3345701420142548TruSeq Adapter, Index 3 (97% over 45bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC2575371.3000271982144078Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC2536771.2805422116489529No Hit
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG2165411.0930825067021286No Hit
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG1159090.5850998206775485No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGGGGG1128100.5694563042613968TruSeq Adapter, Index 3 (97% over 45bp)
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC1077160.5437421795037729Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC967060.4881645364763996No Hit
TGATCTGAACTTTTTATCACATTTAAGCCAGATCGGAAGAGCACACGTCT851460.4298105352596481Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC755630.3814362797527163No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC669290.3378525041034574Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA639530.32282988233693033Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC474660.2396047596673297No Hit
TGTCTGTCGGCATTTGACGGCATCTGCCACAGATCGGAAGAGCACACGTC358540.18098826640358234No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGTG315890.15945887062594863TruSeq Adapter, Index 3 (97% over 45bp)
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC308470.15571331103227823Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATCTGAACTTTTTATCACATTGCAACCAAGATCGGAAGAGCACACGTC298750.15080672892304964No Hit
TGATCTGAACTTTTTATCACATTGCAACCAGATCGGAAGAGCACACGTCT291930.14736404476822054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGTTGTGTTTCTTTCGGTTGTAAAAGCAGATCGGAAGAGCACACGTCT271410.13700570475984905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCAGGACATTCAATTCTCTGGCAAGACTGAGATCGGAAGAGCACACGTC268420.1354963754896234No Hit
TGATCTGAACTTTTTATCACATTTAAGCTAGATCGGAAGAGCACACGTCT265260.13390123151172603Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT261070.1317861513638178No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTCAGATCGGAAGAGCACACGTCT259970.13123087972594216Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG259760.1311248733223477No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTCATAGATCGGAAGAGCACACGT253210.12781848311499716No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGGGTG245360.1238558627901572TruSeq Adapter, Index 3 (97% over 45bp)
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC234160.1182021879317868No Hit
TGGATCCAATAGAAATTATCTGTTCATTAGATCGGAAGAGCACACGTCTG229530.11586499912872833Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTAGAACTGTCTTACTATCTTGTAGAGCAGATCGGAAGAGCACACGTC228710.1154510693623119No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTTAGATCGGAAGAGCACACGTC214630.10834359239750344No Hit
TATCACAGCTGGCTTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTC214510.10828301730973516Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC214310.1081820588301214No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGGGGGGGG212160.10709675517427351TruSeq Adapter, Index 3 (97% over 41bp)
TAAGATCTCTAGATTATCCATTTTGTCTGAGATCGGAAGAGCACACGTCT201690.10181157876649333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)