FastQCFastQC Report
Thu 5 Dec 2024
26_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17972540
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA6619713.683235647270781Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4882172.716460778498754Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG2965761.6501618580345352Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC2124151.1818863666460055No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1026830.5713327109022988Illumina RNA PCR Primer (100% over 50bp)
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG855220.4758481550187119Illumina Small RNA Adapter 1 (100% over 26bp)
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA780890.4344906173529173Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT593820.33040404973364923No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT526700.2930581876573929Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG499130.2777181188635552No Hit
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG347870.19355639214045428Illumina DpnII expression Sequencing Primer (96% over 30bp)
CACGAGCCGAGTGATCCACCGCATAGAGTTGGATCGTCGGACTGTAGAAC319630.17784353241111162No Hit
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT293870.1635105555475186No Hit
CATGCCATCCAGATGGCGAACAGAGACAAAGATCGTCGGACTGTAGAACT292640.1628261781584573No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC279570.15555397289420417No Hit
GATTGTCGAGAGATGACAAGTACAGTCGTAGATCGTCGGACTGTAGAACT271210.15090243226611263No Hit
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC259790.1445482942310881Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC254480.1415937869661161No Hit
ACTAAAGGTAGTCCAGTCCTGAGTAGATAGATCGTCGGACTGTAGAACTC251080.13970201206952385Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATATTCACAGATCCTGATGATATAGCCAGATCGTCGGACTGTAGAACTCT249120.13861145948207654Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTTCAATTACTTCAAAGAAAACTAGACCAGATCGTCGGACTGTAGAACT249110.13860589543826304No Hit
AAAAATATAGGTCAAAGTCAATTACGTCAAGATCGTCGGACTGTAGAACT218470.1215576651936788No Hit
AAATTGTTGAAGCTTTAGACCGAGTATTCAGATCGTCGGACTGTAGAACT217980.12128502704681697No Hit
CCTGGATAAATGAGCTCATCTAGAAGTCGAGATCGTCGGACTGTAGAACT205790.11450245763815242No Hit
AATATAATTGATTCTACACAACAATATACTTAAGATCGTCGGACTGTAGA204590.11383477238053163No Hit
AACAGTACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG192270.10697987040229148Illumina RNA PCR Primer (100% over 36bp)
GCTGTGAAGAAGGAGAGCAGTCTGGTTTTTAGATCGTCGGACTGTAGAAC190090.10576690885094706No Hit
CAATGGCATCAAGATCTTCAACGTCTTCAGATCGTCGGACTGTAGAACTC189720.1055610392298473Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACATAAGTTGGAATATGACAGATCTGAGATCGTCGGACTGTAGAACTCTG187790.10448717877384053Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCTTTTTCAGAGATGTAGGAGATCATTGAGATCGTCGGACTGTAGAACTC187110.10410882379452209Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACGGTGCATACACGTCCTGACAAGACTTGGATCGTCGGACTGTAGAACT186960.1040253631373195No Hit
AAGAGACTACAGTATCCTAGACACTATGAGATCGTCGGACTGTAGAACTC183100.10187764222530594Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)