FastQCFastQC Report
Thu 5 Dec 2024
26_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17972540
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6339753.52746467666785Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC2824601.5716198155630756Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG2071581.1526361883184013No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGTATG1597760.8890006643468314TruSeq Adapter, Index 2 (97% over 49bp)
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC871260.4847728812955765Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA785280.4369332325870467Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGTGTG633620.3525489441114055TruSeq Adapter, Index 2 (97% over 46bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG580520.32300387146168547No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC504350.28062254973420564Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC489100.27213738291860806No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGGGTG470770.2619384906084504TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGGATG462100.2571144646221402TruSeq Adapter, Index 2 (97% over 45bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC425760.23689472940385722No Hit
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC347950.19360090449096232Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGGGGG329800.18350216496944785TruSeq Adapter, Index 2 (97% over 45bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT309570.17224610433472395No Hit
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC284130.15809117687316318No Hit
TTTGTCTCTGTTCGCCATCTGGATGGCATGAGATCGGAAGAGCACACGTC281270.1564998603425003No Hit
TACGACTGTACTTGTCATCTCTCGACAATCAGATCGGAAGAGCACACGTC272060.1513753759902607No Hit
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT271920.1512974793768716No Hit
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG257390.1432129237158465No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGGGGG250730.1395072705360511TruSeq Adapter, Index 2 (97% over 45bp)
TAAGATCTCTAGATTATCCATTTTGTCTGAGATCGGAAGAGCACACGTCT248580.1383110011161472Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCTACTCAGGACTGGACTACCTTTAGTAGATCGGAAGAGCACACGTCT241280.13424924913228736Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTCTAGTTTTCTTTGAAGTAATTGAAGTAGATCGGAAGAGCACACGTC239460.13323659315822917No Hit
TGGCTATATCATCAGGATCTGTGAATATAGATCGGAAGAGCACACGTCTG238290.1325856000320489Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAATACTCGGTCTAAAGCTTCAACAATTTAGATCGGAAGAGCACACGTC211960.11793547267108599No Hit
TTGACGTAATTGACTTTGACCTATATTTTTAGATCGGAAGAGCACACGTC210800.11729004358871924No Hit
TCGACTTCTAGATGAGCTCATTTATCCAGGAGATCGGAAGAGCACACGTC198640.11052416631149521No Hit
TAAAAACCAGACTGCTCTCCTTCTTCACAGCAGATCGGAAGAGCACACGT193520.10767537587897984No Hit
TTAAGTATATTGTTGTGTAGAATCAATTATATTAGATCGGAAGAGCACAC188870.10508809550569927No Hit
TGAAGACGTTGAAGATCTTGATGCCATTGAGATCGGAAGAGCACACGTCT182210.10138244232590385Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTACTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA182020.10127672549344724TruSeq Adapter, Index 2 (100% over 40bp)
CAAGTCTTGTCAGGACGTGTATGCACCGTTAGATCGGAAGAGCACACGTC181620.10105416374090695No Hit
TCAGATCTGTCATATTCCAACTTATGTAGATCGGAAGAGCACACGTCTGA181100.10076483346260462Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAATGATCTCCTACATCTCTGAAAAAGGAGATCGGAAGAGCACACGTCT180040.10017504481837292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)