FastQCFastQC Report
Thu 5 Dec 2024
260_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename260_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19282887
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA7441423.859079815174979Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4460032.3129472262115107Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG3336731.7304099743985433Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC1882580.9762957175447846No Hit
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT1406190.7292424625005581No Hit
GGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC974360.5052977803583042Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT953470.4944643403241434No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT943510.48929913866113517Illumina RNA PCR Primer (100% over 50bp)
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA860580.4462920930875133Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG781580.40532312407369286Illumina Small RNA Adapter 1 (100% over 26bp)
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT642830.33336813102726787No Hit
CATTCCATCCAGATGGCGAACCGAGACAAAGATCGTCGGACTGTAGAACT531840.2758093225355726No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG491220.25474401213884623No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT490260.25424616137614664Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCAGGTGTAAGCACCAAAAGACCGATACAGATCGTCGGACTGTAGAACTC394980.20483447317821238Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG385650.19999598607822575Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC376270.19513156925101516Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC313080.16236157998540365Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAAGGCCATTACACGCCACCAGACCAGTGAGATCGTCGGACTGTAGAACT277700.14401370500174585No Hit
ACTTCAATTACTTCAAAGAAAACTAGACCAGATCGTCGGACTGTAGAACT252090.13073249871764533No Hit
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC250860.13009462742793648Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC221900.11507612941983221No Hit
GTATACAATGTGTACGACAGTTTCTCGGAGATCGTCGGACTGTAGAACTC220320.11425675003955581Illumina DpnII expression Sequencing Primer (100% over 21bp)
CACGAGCCGAGTGATCCACCGCATAGAGTTGGATCGTCGGACTGTAGAAC205310.10647264592692993No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC203120.1053369238745215No Hit
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG199910.10367223538674473Illumina Small RNA Adapter 1 (100% over 26bp)
CATGTTTTTATGAAACAAACACAGACCTTAGATCGTCGGACTGTAGAACT198660.1030239922061463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)