FastQCFastQC Report
Thu 5 Dec 2024
260_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename260_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19282887
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7036923.649308321933329Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3138621.62767120919186Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTGGGGGG2460191.275841112381149TruSeq Adapter, Index 12 (97% over 45bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG1830080.9490695039596508No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTAGGGGG1827600.9477833894893435TruSeq Adapter, Index 12 (97% over 45bp)
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC1463470.7589475580083004No Hit
TGATCTGAACTTTTTATCACATTTAAGCCAGATCGGAAGAGCACACGTCT1010790.5241901796136648Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG930330.4824640625649053No Hit
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA876700.4546518371445106Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC809430.4197659821374258Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC569760.2954744276622064No Hit
TTTGTCTCGGTTCGCCATCTGGATGGAATGAGATCGGAAGAGCACACGTC510080.2645247052477152No Hit
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC480230.24904465809502488No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC464170.24071602971069633Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC419380.2174881800634936No Hit
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC390560.20254228529161633Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTGGGGGGGGGGG376620.1953130773415827TruSeq Adapter, Index 12 (97% over 41bp)
TGTATCGGTCTTTTGGTGCTTACACCTGGAGATCGGAAGAGCACACGTCT374260.1940891942166129Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTGAACTTTTTATCACATTGCAACCAGATCGGAAGAGCACACGTCT323830.16793647133855008Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTGAACTTTTTATCACATTTAAGCTAGATCGGAAGAGCACACGTCT316700.16423889223641666Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACTGGTCTGGTGGCGTGTAATGGCCTTCAGATCGGAAGAGCACACGTC272180.1411510631162232No Hit
TGGTCTAGTTTTCTTTGAAGTAATTGAAGTAGATCGGAAGAGCACACGTC239050.12397002585764258No Hit
TAAGATCTCTAGATTATCCATTTTGTCTGAGATCGGAAGAGCACACGTCT237710.12327510916804107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGAGAAACTGTCGTACACATTGTATACAGATCGGAAGAGCACACGTCT230930.1197590381564752Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG227570.11801656048702666No Hit
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC202750.10514504389306435No Hit
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT198160.10276469493390696No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT198120.10274395115212778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)