FastQCFastQC Report
Thu 5 Dec 2024
24_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename24_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19061228
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA7568623.970688562143006Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG3718521.950829191067858Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3446411.8080734357723438Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC2120351.1123889814444274No Hit
GCTATAAAGACAACTTTGCAGACCAAATAGATCGTCGGACTGTAGAACTC1777220.9323743465006556Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG906370.4755045162882475Illumina Small RNA Adapter 1 (100% over 26bp)
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA826350.43352401010050345Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG759210.3983006761159354No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT731420.38372134261234375No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT709750.37235271515560275Illumina RNA PCR Primer (100% over 50bp)
CCAGGTGTAAGCACCAAAAGACCGATACAGATCGTCGGACTGTAGAACTC516240.2708324983049361Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAATTGTTGAAGCTTTAGACCGAGTATTCAGATCGTCGGACTGTAGAACT481840.2527853924206772No Hit
AACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG413880.21713186579584484Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG403190.21152362271727718Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA392000.20565306705318248Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGGAATTATCTATTTCCCAGAAGATCACAGATCGTCGGACTGTAGAACT384370.2016501769980402No Hit
ACTAAAGGTAGTCCAGTCCTGAGTAGATAGATCGTCGGACTGTAGAACTC382580.2007110979418535Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTATAAAGACAACTTTGCAGACCAAATAGATCGTCGGACTGTAGAACTC364490.19122062859748595Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAAGCTCTAAAAACAGCCAAGAGATCAGAGATCGTCGGACTGTAGAACTC353750.1855861542603656Illumina DpnII expression Sequencing Primer (100% over 21bp)
CATGCCATCCAGATGGCGAACAGAGACAAAGATCGTCGGACTGTAGAACT331170.17374011789796545No Hit
GAAGAGGTTCTCTCTATAGCCCGGACATAGATCGTCGGACTGTAGAACTC318510.16709836323242133Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT307150.16113862128924747Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC274070.14378402063077994No Hit
GTATACAATGTGTACAACAGTTTCTCGGAGATCGTCGGACTGTAGAACTC263530.1382544713278704Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT260940.13689569213484043No Hit
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC231440.12141924958874632No Hit
AACTTCAAATATCAGAGAGAACATGGAAAAGATCGTCGGACTGTAGAACT218350.11455190609964898No Hit
AACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTA212040.111241521270298Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATATAATTGATTCTACACAACAATATACTTAAGATCGTCGGACTGTAGA199860.10485158668685984No Hit
CAGTCTTGAAGTCCTGAGCGATTTCACGTAGATCGTCGGACTGTAGAACT196520.10309933861553935No Hit
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG193730.10163563438829859Illumina Small RNA Adapter 1 (100% over 26bp)
CCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG191760.10060212280132214Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)