FastQCFastQC Report
Thu 5 Dec 2024
24_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename24_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19061228
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7230373.793234098033978Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3534271.8541670033011513Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGG3262521.711600113067217TruSeq Adapter, Index 1 (97% over 45bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG2082331.092442732440953No Hit
TATTTGGTCTGCAAAGTTGTCTTTATAGCAGATCGGAAGAGCACACGTCT1643240.8620850660828359Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC879290.4612976666561042Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA807000.4233725130406079Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC749210.39305442440539506No Hit
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG721110.37831245709877664No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGGGGGGG584800.30680080003240084TruSeq Adapter, Index 1 (97% over 41bp)
TGTATCGGTCTTTTGGTGCTTACACCTGGAGATCGGAAGAGCACACGTCT480460.25206140968462265Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAATACTCGGTCTAAAGCTTCAACAATTTAGATCGGAAGAGCACACGTC465790.2443651584252599No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC434630.2280178380952161No Hit
TATTTGGTCTGCAAAGTTGTCTTTATAGTAGATCGGAAGAGCACACGTCT426440.22372115794428354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTAGGAGATTTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC395640.2075627026758192Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC390520.20487662180002256Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATCTACTCAGGACTGGACTACCTTTAGTAGATCGGAAGAGCACACGTCT371880.1950976086115753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTAGGAGATTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA367230.19265810156617402Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGATCTTCTGGGAAATAGATAATTCCGTAGATCGGAAGAGCACACGTC366570.19231184895327835No Hit
TCTGATCTCTTGGCTGTTTTTAGAGCTTGAGATCGGAAGAGCACACGTCT338440.1775541428915283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATGTCCGGGCTATAGAGAGAACCTCTTCAGATCGGAAGAGCACACGTCT317090.1663533954895246Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGTCTCTGTTCGCCATCTGGATGGCATGAGATCGGAAGAGCACACGTC314010.16473754996267817No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC294380.1544391578548874Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT268860.1410507234895884No Hit
TCCGAGAAACTGTTGTACACATTGTATACAGATCGGAAGAGCACACGTCT261310.1370898034481304Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC250880.1316179629140368No Hit
TATCACAGCTGGCTTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTC218890.11483520369201816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG215360.11298327683819741No Hit
TTTTCCATGTTCTCTCTGATATTTGAAGTTAGATCGGAAGAGCACACGTC208940.1096151832400305No Hit
TTCGACTAGGAGATTTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT202020.10598477705633655Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)