FastQCFastQC Report
Thu 5 Dec 2024
211_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename211_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17846428
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA7212304.041312917072257Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG3606552.0208805930239935Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3105881.7403370579255413Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC1795001.005803514294289No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG997100.5587112446255351Illumina Small RNA Adapter 1 (100% over 26bp)
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT962960.5395813660862555No Hit
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA781750.4380428397212036Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT654330.36664479861180066Illumina RNA PCR Primer (100% over 50bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT631660.3539419765120505No Hit
GGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC629010.3524570855299447Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG526150.29482090197545413No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT485990.2723177993938059Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT413380.23163178648410765No Hit
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG384500.21544927646025303Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT364280.2041192780986761No Hit
AATATAATTGATTCTACACAACAATATACTTAAGATCGTCGGACTGTAGA296610.16620132611411093No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC283840.15904583258902005No Hit
AAATGATCAAAGTTACATGATTTAGACACAGATCGTCGGACTGTAGAACT282140.1580932610155937No Hit
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG249600.13985992042777412Illumina Small RNA Adapter 1 (100% over 26bp)
ACCTGTTGGAAAGTCTACAGATCTAGTTCAGATCGTCGGACTGTAGAACT249500.13980388680580785No Hit
AGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC229400.1285411287905905Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC222180.12449550128462682Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTGCTCTTGTAATGAGGAGCTTCAGACCTTAGATCGTCGGACTGTAGAAC215050.12050030403843279No Hit
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC196240.1099603797465801No Hit
ACTTGACTCAGTAGAGAGTGATATTCCCGAGATCGTCGGACTGTAGAACT189000.10590354551622319No Hit
AATTGATTCTACACAACAATATACTTAAGATCGTCGGACTGTAGAACTCT182940.1025079080250681Illumina DpnII expression Sequencing Primer (100% over 22bp)
CATGCCATCCAGATGGCGAACCGAGACAAAGATCGTCGGACTGTAGAACT180430.10110146411371507No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)