FastQCFastQC Report
Thu 5 Dec 2024
211_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename211_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17846428
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6946773.892526840665258Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3453831.9353060455571276Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG1749580.9803530431972157No Hit
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC1038870.5821164885208402Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC994490.5572487670922159No Hit
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA798750.44756855545546703Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGGGGG696790.3904366744986728TruSeq Adapter, Index 11 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGGGTG680130.3811014730790946TruSeq Adapter, Index 11 (97% over 45bp)
TGATCTGAACTTTTTATCACATTTAAGCCAGATCGGAAGAGCACACGTCT645680.3617978903117195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG618900.34679208634915626No Hit
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC515900.28907745572391297No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC470590.2636886216110025No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC467220.26180028855073967Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC389180.21807164996827375Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC382920.21456394523318617No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGGATG371190.2079912013765444TruSeq Adapter, Index 11 (97% over 45bp)
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC354040.19838143520933155No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTATG282250.15815489799975657TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTGTG279250.15647388934076892TruSeq Adapter, Index 11 (97% over 46bp)
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT278120.15584070941255024No Hit
TTAAGTATATTGTTGTGTAGAATCAATTATATTAGATCGGAAGAGCACAC276760.15507865215380914No Hit
TGTGTCTAAATCATGTAACTTTGATCATTTAGATCGGAAGAGCACACGTC273530.1532687661642991No Hit
TGAACTAGATCTGTAGACTTTCCAACAGGTAGATCGGAAGAGCACACGTC241600.13537723067047366No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTGGGGGG229410.12854673215278709TruSeq Adapter, Index 11 (97% over 45bp)
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC224330.12570022415690132No Hit
TAAGGTCTGAAGCTCCTCATTACAAGAGCACAGATCGGAAGAGCACACGT224010.1255209165666093No Hit
TATCACAGCTGGCTTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTC223690.1253416089763173Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAGATCTCTAGATTATCCATTTTGTCTGAGATCGGAAGAGCACACGTCT213990.1199063476455905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTGAACTTTTTATCACATTTAAGCTAGATCGGAAGAGCACACGTCT205630.1152219368492115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG198830.11141165055550611No Hit
TCGGGAATATCACTCTCTACTGAGTCAAGTAGATCGGAAGAGCACACGTC184470.10336522244115182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)