FastQCFastQC Report
Thu 5 Dec 2024
199_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename199_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16783759
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA7161834.267119183491612Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG3430812.044124918619244Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2854071.7004951036296456Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC1877321.1185336967719806No Hit
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT974170.5804242065201246No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG886900.5284275113816875Illumina Small RNA Adapter 1 (100% over 26bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT816830.486678818493521No Hit
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA794990.4736662388920146Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT750330.44705718188637006Illumina RNA PCR Primer (100% over 50bp)
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT569910.33956040479370564Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG532340.3171756696458761No Hit
GGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC471560.2809620896010244Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT438930.2615206760297261No Hit
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG378600.22557521232281758Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG238980.14238764987032998Illumina Small RNA Adapter 1 (100% over 26bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC226150.13474335516852928No Hit
ATGAAGAGGTTCTCTCTATAGCCCGGACATAGATCGTCGGACTGTAGAAC222660.13266396401425928No Hit
GAAGAGGTTCTCTCTATAGCCCGGACATAGATCGTCGGACTGTAGAACTC221460.1319489871130776Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTGCTCTTGTAATGAGGAGCTTCAGACCTTAGATCGTCGGACTGTAGAAC218380.13011387973337796No Hit
GACTTGTTCATATTGAGTTATTCAGATCTGAGATCGTCGGACTGTAGAAC211620.12608617652338788No Hit
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC208750.12437619010139504No Hit
AATCAGCAAAACAGTCACAAATTAAATCAGATCGTCGGACTGTAGAACTC194320.11577859286468545Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATATAATTGATTCTACACAACAATATACTTAAGATCGTCGGACTGTAGA192980.11498020199169924No Hit
ACAAATCTCCTAGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT192090.11444992745665615Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCTTCATAAAGAACACAAGAGACATAGAGATCGTCGGACTGTAGAACT186260.11097633134508189No Hit
GACATACAGAGGGATGCATGAACAGACATAGATCGTCGGACTGTAGAACT184630.11000515438764344No Hit
TCTCAGACAAATGCCTCTGATAACATCAAGAATGATCGTCGGACTGTAGA180520.10755635850109621No Hit
AGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC177340.10566166971296478Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)