FastQCFastQC Report
Thu 5 Dec 2024
199_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename199_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16783759
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6870064.0932785081101315Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3269791.94818693476235Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG1818761.083642823994315No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGGGGG1249790.7446424844398682TruSeq Adapter, Index 10 (97% over 45bp)
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC1023230.6096548454967686No Hit
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC924930.5510863210083033Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA814260.48514757629682365Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG795010.47367815517370093No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGGGG664680.3960257055645282TruSeq Adapter, Index 10 (97% over 45bp)
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC547490.3262022530232947Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC518550.30895939342313006No Hit
TGATCTGAACTTTTTATCACATTTAAGCCAGATCGGAAGAGCACACGTCT492550.2934682272308605Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC416830.248353184766297No Hit
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC396720.2363713635306608No Hit
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC385730.2298233667440053Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAAGGTCTGAAGCTCCTCATTACAAGAGCACAGATCGGAAGAGCACACGT231620.13800245820974907No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGTGGG230820.13752580694229463TruSeq Adapter, Index 10 (97% over 46bp)
TATGTCCGGGCTATAGAGAGAACCTCTTCAGATCGGAAGAGCACACGTCT227320.1354404576471814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT218950.13045349376143925No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTCATAGATCGGAAGAGCACACGT216930.12924994931111677No Hit
TCAGATCTGAATAACTCAATATGAACAAGTCAGATCGGAAGAGCACACGT216510.12899970739570318No Hit
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG212790.12678327900204No Hit
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC202520.12066426835609352No Hit
TATCACAGCTGGCTTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTC199320.11875766328627572Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGGGTG197580.11772094677956232TruSeq Adapter, Index 10 (97% over 45bp)
TATGTCTGTTCATGCATCCCTCTGTATGTCAGATCGGAAGAGCACACGTC187580.11176280593638172No Hit
TGATTTAATTTGTGACTGTTTTGCTGATTAGATCGGAAGAGCACACGTCT186790.11129211280977046Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC184520.10993961483836845Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTAAGTATATTGTTGTGTAGAATCAATTATATTAGATCGGAAGAGCACAC179040.10667455365630549No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATTAGATCGGAAGAGCACACGTC178800.10653155827606915No Hit
ATTCTTGATGTTATCAGAGGCATTTGTCTGAGAAGATCGGAAGAGCACAC175070.10430917174156279No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)