FastQCFastQC Report
Thu 5 Dec 2024
196_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename196_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22003473
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA11217315.0979724882521955Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG5512092.5050999903515234Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3591171.6320923519664372Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC3072651.3964386440267862No Hit
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT1720700.7820129122343551No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT1313010.5967285255377639No Hit
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1250660.5683920897396516Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG860380.3910200903284677No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT791820.35986137279328584Illumina RNA PCR Primer (100% over 50bp)
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT745580.33884650845800574No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG696090.3163546045662882Illumina Small RNA Adapter 1 (100% over 26bp)
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG609200.2768653839328001Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTATAAAGACAACTTTGCAGACCAAATAGATCGTCGGACTGTAGAACTC579760.26348567792002653Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT539710.24528400584762233Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC456350.2073990774092799Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTTTTACAACCGAAAGAAACACAACGTAGATCGTCGGACTGTAGAACTC418470.19018361328686612Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTCCTAGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT355390.16151541168069242Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGT338060.15363938229205906Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC333880.15173968218562586No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC315070.14319103170667646No Hit
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC280530.12749350977457058Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTCTCGTGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA274260.1246439596149208No Hit
AATATGATTGATTCTACACAACAATATACTTAAGATCGTCGGACTGTAGA264860.12037190674399446No Hit
CAATCGCATCAAGATCTTCAACGTCTTCAGATCGTCGGACTGTAGAACTC242990.11043256671344563Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT233890.10629685595542122No Hit
GGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC224310.10194299781675376Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)