FastQCFastQC Report
Thu 5 Dec 2024
196_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename196_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22003473
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10736724.879556968120442Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC5239032.38100139918821Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGG3146141.4298379169506559TruSeq Adapter, Index 9 (97% over 45bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG2994291.3608260841368087No Hit
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC1752430.7964333630422797No Hit
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG1283550.583339730050797No Hit
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1256310.5709598661993041Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC842060.3826941319672581No Hit
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC716360.32556678666136024Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC705040.3204221442678617No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC616630.2802421235956705No Hit
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC607860.27625638916183826Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATTTGGTCTGCAAAGTTGTCTTTATAGCAGATCGGAAGAGCACACGTCT573600.26068611986844076Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC515380.2342266604912779Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCTGAACTTTTTATCACATTTAAGCCAGATCGGAAGAGCACACGTCT463570.21068037759311903Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGTTGTGTTTCTTTCGGTTGTAAAAGCAGATCGGAAGAGCACACGTCT415740.1889429000594588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC341430.15517095869365713Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG336140.1527667927694869No Hit
CTTTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG323190.14688135822922135Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT305660.13891443409865342No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGGGGGGG289050.13136562578098468TruSeq Adapter, Index 9 (97% over 41bp)
TAAGATCTCTAGATTATCCATTTTGTCTGAGATCGGAAGAGCACACGTCT270600.12298058583751757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAGTATATTGTTGTGTAGAATCAATCATATTAGATCGGAAGAGCACAC248870.11310487212632296No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCACGAGACTAGATCGGAAGAGCACAC248420.11290035895697012No Hit
TGAAGACGTTGAAGATCTTGATGCGATTGAGATCGGAAGAGCACACGTCT232970.10587874014252203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTGAACTTTTTATCACATTGCAACCAGATCGGAAGAGCACACGTCT227290.10329732947157932Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)