WARNING An augustus species is mentioned in the config file, dataset default species (fly) will be ignored INFO ****************** Start a BUSCO 3.0.2 analysis, current time: 07/21/2021 10:37:23 ****************** INFO Configuration loaded from /gscratch/scrubbed/samwhite/outputs/20210721_pcom_busco_cnidaria_transcriptome_v2.0/config.ini INFO Init tools... INFO Check dependencies... INFO Check input file... INFO To reproduce this run: python /gscratch/srlab/programs/busco-v3/scripts/run_BUSCO.py -i /gscratch/srlab/sam/data/P_compressa/transcriptomes/Cnidaria_MEGAN-extractions.fasta -o Cnidaria_MEGAN-extractions.fasta -l /gscratch/srlab/sam/data/databases/BUSCO/metazoa_odb9/ -m transcriptome -c 40 --long -z INFO Mode is: transcriptome INFO The lineage dataset is: metazoa_odb9 (eukaryota) INFO Temp directory is ./tmp/ INFO ****** Step 1/2, current time: 07/21/2021 10:37:25 ****** INFO Create blast database... INFO [makeblastdb] Building a new DB, current time: 07/21/2021 10:37:25 INFO [makeblastdb] New DB name: /gscratch/scrubbed/samwhite/outputs/20210721_pcom_busco_cnidaria_transcriptome_v2.0/tmp/Cnidaria_MEGAN-extractions.fasta_560747313 INFO [makeblastdb] New DB title: /gscratch/srlab/sam/data/P_compressa/transcriptomes/Cnidaria_MEGAN-extractions.fasta INFO [makeblastdb] Sequence type: Nucleotide INFO [makeblastdb] Keep MBits: T INFO [makeblastdb] Maximum file size: 1000000000B INFO [makeblastdb] Adding sequences from FASTA; added 261540 sequences in 8.50407 seconds. INFO [makeblastdb] 1 of 1 task(s) completed at 07/21/2021 10:37:34 INFO Running tblastn, writing output to /gscratch/scrubbed/samwhite/outputs/20210721_pcom_busco_cnidaria_transcriptome_v2.0/run_Cnidaria_MEGAN-extractions.fasta/blast_output/tblastn_Cnidaria_MEGAN-extractions.fasta.tsv... INFO [tblastn] Warning: [tblastn] Number of threads was reduced to 28 to match the number of available CPUs INFO [tblastn] 1 of 1 task(s) completed at 07/21/2021 10:41:48 INFO ****** Step 2/2, current time: 07/21/2021 10:41:48 ****** INFO Maximum number of candidate transcript per BUSCO limited to: 3 INFO Getting coordinates for candidate transcripts... INFO Extracting candidate transcripts... INFO Translating candidate transcripts... INFO Running HMMER to confirm transcript orthology: INFO [hmmsearch] 244 of 2438 task(s) completed at 07/21/2021 10:42:28 INFO [hmmsearch] 488 of 2438 task(s) completed at 07/21/2021 10:42:29 INFO [hmmsearch] 2438 of 2438 task(s) completed at 07/21/2021 10:42:33 INFO Results: INFO C:94.1%[S:31.3%,D:62.8%],F:2.9%,M:3.0%,n:978 INFO 920 Complete BUSCOs (C) INFO 306 Complete and single-copy BUSCOs (S) INFO 614 Complete and duplicated BUSCOs (D) INFO 28 Fragmented BUSCOs (F) INFO 30 Missing BUSCOs (M) INFO 978 Total BUSCO groups searched INFO BUSCO analysis done with WARNING(s). Total running time: 315.8285369873047 seconds INFO Results written in /gscratch/scrubbed/samwhite/outputs/20210721_pcom_busco_cnidaria_transcriptome_v2.0/run_Cnidaria_MEGAN-extractions.fasta/