CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P01-AGTTCC-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P01-AGTTCC-READ2-Sequences.txt.gz_val_2.fq.gz) -o male_bleached_K5_01 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { male_bleached_K5_01 } ### [ threads ] => { 23 } Logs will be written to male_bleached_K5_01/logs [2019-03-14 07:46:19.860] [jointLog] [info] parsing read library format [2019-03-14 07:46:19.860] [jointLog] [info] There is 1 library. [2019-03-14 07:46:19.927] [stderrLog] [info] Loading Suffix Array [2019-03-14 07:46:19.927] [jointLog] [info] Loading Quasi index [2019-03-14 07:46:19.927] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 07:46:19.964] [stderrLog] [info] Loading Transcript Info [2019-03-14 07:46:19.973] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 07:46:19.977] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 07:46:19.979] [stderrLog] [info] Computing transcript lengths [2019-03-14 07:46:19.979] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 07:46:20.330] [jointLog] [info] done [2019-03-14 07:46:20.330] [jointLog] [info] Index contained 5838 targets [2019-03-14 07:46:20.330] [stderrLog] [info] Done loading index  processed 500000 fragments hits: 96066, hits per frag: 0.212992 processed 1000000 fragments hits: 192072, hits per frag: 0.200424 processed 1500000 fragments hits: 287711, hits per frag: 0.206113 processed 2000000 fragments hits: 384028, hits per frag: 0.197979 processed 2500000 fragments hits: 480047, hits per frag: 0.199213 processed 3000001 fragments hits: 574864, hits per frag: 0.197498 processed 3500000 fragments hits: 671007, hits per frag: 0.19518 processed 4000000 fragments hits: 767880, hits per frag: 0.195039 processed 4500000 fragments hits: 863815, hits per frag: 0.195537 processed 5000001 fragments hits: 958239, hits per frag: 0.19511 processed 5500000 fragments hits: 1053764, hits per frag: 0.19337 processed 6000000 fragments hits: 1149165, hits per frag: 0.193888 processed 6500001 fragments hits: 1244434, hits per frag: 0.192663 processed 7000000 fragments hits: 1340581, hits per frag: 0.193768 processed 7500000 fragments hits: 1435726, hits per frag: 0.19212 processed 8000000 fragments hits: 1532433, hits per frag: 0.192887 processed 8500000 fragments hits: 1628303, hits per frag: 0.192561 processed 9000000 fragments hits: 1723516, hits per frag: 0.19219 processed 9500000 fragments hits: 1817983, hits per frag: 0.191676 processed 10000000 fragments hits: 1913265, hits per frag: 0.193999 processed 10500001 fragments hits: 2008622, hits per frag: 0.192309 processed 11000001 fragments hits: 2104664, hits per frag: 0.193163 processed 11500000 fragments hits: 2200022, hits per frag: 0.191781 processed 12000001 fragments hits: 2295251, hits per frag: 0.191826 processed 12500001 fragments hits: 2389899, hits per frag: 0.192561 processed 13000000 fragments hits: 2485752, hits per frag: 0.192438 processed 13500001 fragments hits: 2581786, hits per frag: 0.191361 processed 14000000 fragments hits: 2676220, hits per frag: 0.192647 processed 14500001 fragments hits: 2772463, hits per frag: 0.191469 processed 15000000 fragments hits: 2867750, hits per frag: 0.193062 processed 15500000 fragments hits: 2963200, hits per frag: 0.192331 processed 16000000 fragments hits: 3059628, hits per frag: 0.191296 processed 16500000 fragments hits: 3156089, hits per frag: 0.191352 processed 17000000 fragments hits: 3252103, hits per frag: 0.191719 processed 17500000 fragments hits: 3347030, hits per frag: 0.192141 processed 18000000 fragments hits: 3442740, hits per frag: 0.191859 processed 18500001 fragments hits: 3537853, hits per frag: 0.191696 processed 19000001 fragments hits: 3632693, hits per frag: 0.191327 processed 19500000 fragments hits: 3727656, hits per frag: 0.191474 processed 20000000 fragments hits: 3823206, hits per frag: 0.192124 processed 20500000 fragments hits: 3919085, hits per frag: 0.191576 processed 21000000 fragments hits: 4013677, hits per frag: 0.191815 processed 21500000 fragments hits: 4108598, hits per frag: 0.191839 processed 22000000 fragments hits: 4204170, hits per frag: 0.191573 processed 22500000 fragments hits: 4299725, hits per frag: 0.191244 processed 23000000 fragments hits: 4396028, hits per frag: 0.191792 processed 23500000 fragments hits: 4492384, hits per frag: 0.191473 processed 24000001 fragments hits: 4587392, hits per frag: 0.191569 processed 24500000 fragments hits: 4684371, hits per frag: 0.191593 [2019-03-14 07:47:18.170] [jointLog] [info] Computed 7374 rich equivalence classes for further processing [2019-03-14 07:47:18.170] [jointLog] [info] Counted 2259062 total reads in the equivalence classes [2019-03-14 07:47:18.180] [jointLog] [warning] 0.00989258% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 07:47:18.181] [jointLog] [warning] Only 2259062 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 07:47:18.181] [jointLog] [info] Mapping rate = 9.09192% [2019-03-14 07:47:18.181] [jointLog] [info] finished quantifyLibrary() [2019-03-14 07:47:18.181] [jointLog] [info] Starting optimizer [2019-03-14 07:47:18.185] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 07:47:18.185] [jointLog] [info] iteration = 0 | max rel diff. = 556.424 [2019-03-14 07:47:18.203] [jointLog] [info] iteration = 100 | max rel diff. = 0.118712 [2019-03-14 07:47:18.217] [jointLog] [info] iteration = 200 | max rel diff. = 0.0618691 [2019-03-14 07:47:18.232] [jointLog] [info] iteration = 300 | max rel diff. = 0.0435063 [2019-03-14 07:47:18.246] [jointLog] [info] iteration = 400 | max rel diff. = 0.0267595 [2019-03-14 07:47:18.261] [jointLog] [info] iteration = 500 | max rel diff. = 0.0203617 [2019-03-14 07:47:18.275] [jointLog] [info] iteration = 600 | max rel diff. = 0.0172347 [2019-03-14 07:47:18.291] [jointLog] [info] iteration = 700 | max rel diff. = 0.0139827 [2019-03-14 07:47:18.307] [jointLog] [info] iteration = 800 | max rel diff. = 0.0120669 [2019-03-14 07:47:18.322] [jointLog] [info] iteration = 900 | max rel diff. = 0.012067 [2019-03-14 07:47:18.338] [jointLog] [info] iteration = 1000 | max rel diff. = 0.0101034 [2019-03-14 07:47:18.340] [jointLog] [info] iteration = 1015 | max rel diff. = 0.00970062 [2019-03-14 07:47:18.341] [jointLog] [info] Finished optimizer [2019-03-14 07:47:18.341] [jointLog] [info] writing output [2019-03-14 07:47:18.351] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: male_bleached_K5_01/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl male_bleached_K5_01/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > male_bleached_K5_01/quant.sf.genes CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P06-GCCAAT-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P06-GCCAAT-READ2-Sequences.txt.gz_val_2.fq.gz) -o male_bleached_K5_02 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { male_bleached_K5_02 } ### [ threads ] => { 23 } Logs will be written to male_bleached_K5_02/logs [2019-03-14 07:47:18.593] [jointLog] [info] parsing read library format [2019-03-14 07:47:18.593] [jointLog] [info] There is 1 library. [2019-03-14 07:47:18.659] [stderrLog] [info] Loading Suffix Array [2019-03-14 07:47:18.659] [jointLog] [info] Loading Quasi index [2019-03-14 07:47:18.659] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 07:47:18.695] [stderrLog] [info] Loading Transcript Info [2019-03-14 07:47:18.704] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 07:47:18.708] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 07:47:18.709] [stderrLog] [info] Computing transcript lengths [2019-03-14 07:47:18.710] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 07:47:19.065] [jointLog] [info] done [2019-03-14 07:47:19.065] [jointLog] [info] Index contained 5838 targets [2019-03-14 07:47:19.065] [stderrLog] [info] Done loading index  processed 500000 fragments hits: 84795, hits per frag: 0.18674 processed 1000000 fragments hits: 169913, hits per frag: 0.184434 processed 1500000 fragments hits: 255912, hits per frag: 0.175886 processed 2000000 fragments hits: 340998, hits per frag: 0.176008 processed 2500001 fragments hits: 425525, hits per frag: 0.170547 processed 3000002 fragments hits: 511039, hits per frag: 0.174227 processed 3500001 fragments hits: 595726, hits per frag: 0.173506 processed 4000000 fragments hits: 680675, hits per frag: 0.17046 processed 4500000 fragments hits: 765460, hits per frag: 0.171866 processed 5000001 fragments hits: 850918, hits per frag: 0.171306 processed 5500000 fragments hits: 935795, hits per frag: 0.172257 processed 6000000 fragments hits: 1019647, hits per frag: 0.172154 processed 6500000 fragments hits: 1105021, hits per frag: 0.172529 processed 7000000 fragments hits: 1190098, hits per frag: 0.17046 processed 7500000 fragments hits: 1275445, hits per frag: 0.171056 processed 8000001 fragments hits: 1360199, hits per frag: 0.170801 processed 8500000 fragments hits: 1444878, hits per frag: 0.171958 processed 9000001 fragments hits: 1529985, hits per frag: 0.171704 processed 9500000 fragments hits: 1615325, hits per frag: 0.171822 processed 10000000 fragments hits: 1700341, hits per frag: 0.170946 processed 10500000 fragments hits: 1785783, hits per frag: 0.171738 processed 11000000 fragments hits: 1870724, hits per frag: 0.170966 processed 11500000 fragments hits: 1955949, hits per frag: 0.170489 processed 12000000 fragments hits: 2041448, hits per frag: 0.171299 processed 12500000 fragments hits: 2125170, hits per frag: 0.17029 processed 13000000 fragments hits: 2209826, hits per frag: 0.170977 processed 13500000 fragments hits: 2295152, hits per frag: 0.170751 processed 14000000 fragments hits: 2380815, hits per frag: 0.171423 processed 14500000 fragments hits: 2465802, hits per frag: 0.170692 processed 15000000 fragments hits: 2552193, hits per frag: 0.171013 processed 15500000 fragments hits: 2637118, hits per frag: 0.170493 processed 16000000 fragments hits: 2722924, hits per frag: 0.171198 processed 16500000 fragments hits: 2808596, hits per frag: 0.171512 processed 17000000 fragments hits: 2893297, hits per frag: 0.170524 processed 17500000 fragments hits: 2978075, hits per frag: 0.171132 processed 18000000 fragments hits: 3064254, hits per frag: 0.170982 [2019-03-14 07:48:02.621] [jointLog] [info] Computed 7296 rich equivalence classes for further processing [2019-03-14 07:48:02.621] [jointLog] [info] Counted 1550851 total reads in the equivalence classes [2019-03-14 07:48:02.630] [jointLog] [warning] 0.0114771% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 07:48:02.631] [jointLog] [warning] Only 1550851 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 07:48:02.631] [jointLog] [info] Mapping rate = 8.4437% [2019-03-14 07:48:02.631] [jointLog] [info] finished quantifyLibrary() [2019-03-14 07:48:02.631] [jointLog] [info] Starting optimizer [2019-03-14 07:48:02.636] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 07:48:02.636] [jointLog] [info] iteration = 0 | max rel diff. = 415.309 [2019-03-14 07:48:02.656] [jointLog] [info] iteration = 100 | max rel diff. = 0.123527 [2019-03-14 07:48:02.672] [jointLog] [info] iteration = 200 | max rel diff. = 0.0531559 [2019-03-14 07:48:02.687] [jointLog] [info] iteration = 300 | max rel diff. = 0.0391319 [2019-03-14 07:48:02.702] [jointLog] [info] iteration = 400 | max rel diff. = 0.028226 [2019-03-14 07:48:02.717] [jointLog] [info] iteration = 500 | max rel diff. = 0.0208174 [2019-03-14 07:48:02.731] [jointLog] [info] iteration = 600 | max rel diff. = 0.0169562 [2019-03-14 07:48:02.746] [jointLog] [info] iteration = 700 | max rel diff. = 0.0151658 [2019-03-14 07:48:02.761] [jointLog] [info] iteration = 800 | max rel diff. = 0.0115074 [2019-03-14 07:48:02.775] [jointLog] [info] iteration = 900 | max rel diff. = 0.0113233 [2019-03-14 07:48:02.788] [jointLog] [info] iteration = 987 | max rel diff. = 0.00949143 [2019-03-14 07:48:02.788] [jointLog] [info] Finished optimizer [2019-03-14 07:48:02.788] [jointLog] [info] writing output [2019-03-14 07:48:02.799] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: male_bleached_K5_02/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl male_bleached_K5_02/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > male_bleached_K5_02/quant.sf.genes CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P07-AGTCAA-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P07-AGTCAA-READ2-Sequences.txt.gz_val_2.fq.gz) -o male_bleached_K5_03 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { male_bleached_K5_03 } ### [ threads ] => { 23 } Logs will be written to male_bleached_K5_03/logs [2019-03-14 07:48:03.295] [jointLog] [info] parsing read library format [2019-03-14 07:48:03.295] [jointLog] [info] There is 1 library. [2019-03-14 07:48:03.361] [stderrLog] [info] Loading Suffix Array [2019-03-14 07:48:03.361] [jointLog] [info] Loading Quasi index [2019-03-14 07:48:03.361] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 07:48:03.397] [stderrLog] [info] Loading Transcript Info [2019-03-14 07:48:03.406] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 07:48:03.410] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 07:48:03.412] [stderrLog] [info] Computing transcript lengths [2019-03-14 07:48:03.412] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 07:48:03.765] [jointLog] [info] done [2019-03-14 07:48:03.765] [jointLog] [info] Index contained 5838 targets [2019-03-14 07:48:03.765] [stderrLog] [info] Done loading index  processed 500000 fragments hits: 97477, hits per frag: 0.198503 processed 1000002 fragments hits: 195163, hits per frag: 0.209573 processed 1500000 fragments hits: 294177, hits per frag: 0.212027 processed 2000000 fragments hits: 392754, hits per frag: 0.205193 processed 2500000 fragments hits: 492012, hits per frag: 0.20243 processed 3000000 fragments hits: 590943, hits per frag: 0.203971 processed 3500000 fragments hits: 688351, hits per frag: 0.19885 processed 4000000 fragments hits: 786020, hits per frag: 0.199521 processed 4500000 fragments hits: 883541, hits per frag: 0.199087 processed 5000000 fragments hits: 982284, hits per frag: 0.200351 processed 5500000 fragments hits: 1080394, hits per frag: 0.201546 processed 6000000 fragments hits: 1178530, hits per frag: 0.198076 processed 6500000 fragments hits: 1276360, hits per frag: 0.197087 processed 7000000 fragments hits: 1375152, hits per frag: 0.199352 processed 7500000 fragments hits: 1472015, hits per frag: 0.197689 processed 8000000 fragments hits: 1569404, hits per frag: 0.198224 processed 8500000 fragments hits: 1667327, hits per frag: 0.198076 processed 9000000 fragments hits: 1765715, hits per frag: 0.19713 processed 9500000 fragments hits: 1864146, hits per frag: 0.1966 processed 10000000 fragments hits: 1961607, hits per frag: 0.196788 processed 10500000 fragments hits: 2060578, hits per frag: 0.197121 processed 11000000 fragments hits: 2160379, hits per frag: 0.197761 processed 11500000 fragments hits: 2259469, hits per frag: 0.197901 processed 12000000 fragments hits: 2357404, hits per frag: 0.197107 processed 12500000 fragments hits: 2456268, hits per frag: 0.197174 processed 13000001 fragments hits: 2554295, hits per frag: 0.198021 processed 13500000 fragments hits: 2651927, hits per frag: 0.197314 processed 14000001 fragments hits: 2749706, hits per frag: 0.197669 processed 14500000 fragments hits: 2848426, hits per frag: 0.197297 processed 15000000 fragments hits: 2946038, hits per frag: 0.197842 processed 15500000 fragments hits: 3044545, hits per frag: 0.197913 processed 16000001 fragments hits: 3141972, hits per frag: 0.196709 processed 16500000 fragments hits: 3239889, hits per frag: 0.19703 processed 17000000 fragments hits: 3337558, hits per frag: 0.197349 processed 17500000 fragments hits: 3435793, hits per frag: 0.196886 processed 18000000 fragments hits: 3532758, hits per frag: 0.197042 processed 18500001 fragments hits: 3631334, hits per frag: 0.197028 processed 19000001 fragments hits: 3730508, hits per frag: 0.19693 processed 19500000 fragments hits: 3828512, hits per frag: 0.196857 processed 20000000 fragments hits: 3926560, hits per frag: 0.196805 processed 20500000 fragments hits: 4023847, hits per frag: 0.197628 processed 21000000 fragments hits: 4122201, hits per frag: 0.197306 processed 21500000 fragments hits: 4219809, hits per frag: 0.197338 processed 22000000 fragments hits: 4317908, hits per frag: 0.196904 processed 22500000 fragments hits: 4416325, hits per frag: 0.19691 processed 23000000 fragments hits: 4514895, hits per frag: 0.196867 processed 23500000 fragments hits: 4612622, hits per frag: 0.196399 processed 24000000 fragments hits: 4710745, hits per frag: 0.197007 processed 24500000 fragments hits: 4808010, hits per frag: 0.196476 processed 25000000 fragments hits: 4906337, hits per frag: 0.196793 processed 25500001 fragments hits: 5003743, hits per frag: 0.196339 [2019-03-14 07:49:05.922] [jointLog] [info] Computed 7398 rich equivalence classes for further processing [2019-03-14 07:49:05.922] [jointLog] [info] Counted 2479140 total reads in the equivalence classes [2019-03-14 07:49:05.932] [jointLog] [warning] 0.0136297% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 07:49:05.932] [jointLog] [warning] Only 2479140 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 07:49:05.932] [jointLog] [info] Mapping rate = 9.56952% [2019-03-14 07:49:05.932] [jointLog] [info] finished quantifyLibrary() [2019-03-14 07:49:05.933] [jointLog] [info] Starting optimizer [2019-03-14 07:49:05.938] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 07:49:05.938] [jointLog] [info] iteration = 0 | max rel diff. = 615.834 [2019-03-14 07:49:05.956] [jointLog] [info] iteration = 100 | max rel diff. = 0.117134 [2019-03-14 07:49:05.972] [jointLog] [info] iteration = 200 | max rel diff. = 0.0572327 [2019-03-14 07:49:05.990] [jointLog] [info] iteration = 300 | max rel diff. = 0.037784 [2019-03-14 07:49:06.007] [jointLog] [info] iteration = 400 | max rel diff. = 0.02534 [2019-03-14 07:49:06.025] [jointLog] [info] iteration = 500 | max rel diff. = 0.0214197 [2019-03-14 07:49:06.042] [jointLog] [info] iteration = 600 | max rel diff. = 0.017772 [2019-03-14 07:49:06.060] [jointLog] [info] iteration = 700 | max rel diff. = 0.0152762 [2019-03-14 07:49:06.077] [jointLog] [info] iteration = 800 | max rel diff. = 0.0123923 [2019-03-14 07:49:06.095] [jointLog] [info] iteration = 900 | max rel diff. = 0.0119455 [2019-03-14 07:49:06.112] [jointLog] [info] iteration = 1000 | max rel diff. = 0.0106927 [2019-03-14 07:49:06.117] [jointLog] [info] iteration = 1027 | max rel diff. = 0.00914205 [2019-03-14 07:49:06.117] [jointLog] [info] Finished optimizer [2019-03-14 07:49:06.117] [jointLog] [info] writing output [2019-03-14 07:49:06.128] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: male_bleached_K5_03/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl male_bleached_K5_03/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > male_bleached_K5_03/quant.sf.genes CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P08-CTTGTA-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P08-CTTGTA-READ2-Sequences.txt.gz_val_2.fq.gz) -o female_bleached_K5_04 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { female_bleached_K5_04 } ### [ threads ] => { 23 } Logs will be written to female_bleached_K5_04/logs [2019-03-14 07:49:06.616] [jointLog] [info] parsing read library format [2019-03-14 07:49:06.616] [jointLog] [info] There is 1 library. [2019-03-14 07:49:06.683] [stderrLog] [info] Loading Suffix Array [2019-03-14 07:49:06.683] [jointLog] [info] Loading Quasi index [2019-03-14 07:49:06.683] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 07:49:06.723] [stderrLog] [info] Loading Transcript Info [2019-03-14 07:49:06.733] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 07:49:06.736] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 07:49:06.738] [stderrLog] [info] Computing transcript lengths [2019-03-14 07:49:06.738] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 07:49:07.075] [jointLog] [info] done [2019-03-14 07:49:07.075] [jointLog] [info] Index contained 5838 targets [2019-03-14 07:49:07.075] [stderrLog] [info] Done loading index  processed 500000 fragments hits: 96372, hits per frag: 0.224853 processed 1000000 fragments hits: 192499, hits per frag: 0.219698 processed 1500000 fragments hits: 289387, hits per frag: 0.199702 processed 2000000 fragments hits: 385822, hits per frag: 0.195752 processed 2500000 fragments hits: 481942, hits per frag: 0.195096 processed 3000001 fragments hits: 577884, hits per frag: 0.19851 processed 3500000 fragments hits: 673391, hits per frag: 0.194247 processed 4000000 fragments hits: 770460, hits per frag: 0.197779 processed 4500000 fragments hits: 867691, hits per frag: 0.195097 processed 5000000 fragments hits: 963895, hits per frag: 0.197278 processed 5500000 fragments hits: 1060867, hits per frag: 0.194289 processed 6000000 fragments hits: 1157709, hits per frag: 0.193413 processed 6500000 fragments hits: 1253637, hits per frag: 0.195344 processed 7000000 fragments hits: 1351198, hits per frag: 0.194569 processed 7500000 fragments hits: 1447856, hits per frag: 0.194386 processed 8000000 fragments hits: 1543397, hits per frag: 0.195313 processed 8500002 fragments hits: 1638981, hits per frag: 0.195868 processed 9000000 fragments hits: 1735151, hits per frag: 0.193463 processed 9500000 fragments hits: 1831442, hits per frag: 0.193669 processed 10000000 fragments hits: 1926808, hits per frag: 0.193645 processed 10500000 fragments hits: 2022099, hits per frag: 0.193168 processed 11000001 fragments hits: 2118361, hits per frag: 0.193513 processed 11500000 fragments hits: 2215408, hits per frag: 0.194944 processed 12000000 fragments hits: 2312082, hits per frag: 0.193482 processed 12500000 fragments hits: 2406371, hits per frag: 0.193489 processed 13000000 fragments hits: 2501286, hits per frag: 0.192886 processed 13500000 fragments hits: 2597605, hits per frag: 0.192793 processed 14000000 fragments hits: 2694018, hits per frag: 0.193762 processed 14500000 fragments hits: 2790496, hits per frag: 0.193053 processed 15000001 fragments hits: 2886996, hits per frag: 0.192836 processed 15500000 fragments hits: 2983009, hits per frag: 0.193 processed 16000000 fragments hits: 3078523, hits per frag: 0.193247 processed 16500000 fragments hits: 3174707, hits per frag: 0.194945 processed 17000000 fragments hits: 3270740, hits per frag: 0.193476 processed 17500001 fragments hits: 3366895, hits per frag: 0.193275 processed 18000000 fragments hits: 3463062, hits per frag: 0.192686 processed 18500000 fragments hits: 3558892, hits per frag: 0.193376 processed 19000000 fragments hits: 3655550, hits per frag: 0.193376 processed 19500000 fragments hits: 3751275, hits per frag: 0.194249 processed 20000001 fragments hits: 3847905, hits per frag: 0.192808 processed 20500000 fragments hits: 3944524, hits per frag: 0.19283 processed 21000000 fragments hits: 4040776, hits per frag: 0.192918 processed 21500001 fragments hits: 4137750, hits per frag: 0.193199 processed 22000000 fragments hits: 4233667, hits per frag: 0.193393 processed 22500000 fragments hits: 4329667, hits per frag: 0.192934 processed 23000000 fragments hits: 4426385, hits per frag: 0.192708 processed 23500000 fragments hits: 4522504, hits per frag: 0.19294 processed 24000000 fragments hits: 4618647, hits per frag: 0.193066 processed 24500000 fragments hits: 4714971, hits per frag: 0.192906 processed 25000001 fragments hits: 4812131, hits per frag: 0.192949 processed 25500000 fragments hits: 4908642, hits per frag: 0.192723 processed 26000000 fragments hits: 5004217, hits per frag: 0.193197 processed 26500000 fragments hits: 5101240, hits per frag: 0.192726 processed 27000000 fragments hits: 5197144, hits per frag: 0.193267 processed 27500000 fragments hits: 5292266, hits per frag: 0.192951 processed 28000000 fragments hits: 5389117, hits per frag: 0.192716 [2019-03-14 07:50:15.657] [jointLog] [info] Computed 7401 rich equivalence classes for further processing [2019-03-14 07:50:15.657] [jointLog] [info] Counted 2716216 total reads in the equivalence classes [2019-03-14 07:50:15.667] [jointLog] [warning] 0.0118102% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 07:50:15.668] [jointLog] [warning] Only 2716216 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 07:50:15.668] [jointLog] [info] Mapping rate = 9.59013% [2019-03-14 07:50:15.668] [jointLog] [info] finished quantifyLibrary() [2019-03-14 07:50:15.668] [jointLog] [info] Starting optimizer [2019-03-14 07:50:15.672] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 07:50:15.673] [jointLog] [info] iteration = 0 | max rel diff. = 623.231 [2019-03-14 07:50:15.691] [jointLog] [info] iteration = 100 | max rel diff. = 0.126924 [2019-03-14 07:50:15.707] [jointLog] [info] iteration = 200 | max rel diff. = 0.063358 [2019-03-14 07:50:15.723] [jointLog] [info] iteration = 300 | max rel diff. = 0.0433714 [2019-03-14 07:50:15.738] [jointLog] [info] iteration = 400 | max rel diff. = 0.0310132 [2019-03-14 07:50:15.753] [jointLog] [info] iteration = 500 | max rel diff. = 0.0246222 [2019-03-14 07:50:15.768] [jointLog] [info] iteration = 600 | max rel diff. = 0.0180039 [2019-03-14 07:50:15.783] [jointLog] [info] iteration = 700 | max rel diff. = 0.0152048 [2019-03-14 07:50:15.799] [jointLog] [info] iteration = 800 | max rel diff. = 0.0121776 [2019-03-14 07:50:15.814] [jointLog] [info] iteration = 900 | max rel diff. = 0.0118751 [2019-03-14 07:50:15.829] [jointLog] [info] iteration = 1000 | max rel diff. = 0.0110757 [2019-03-14 07:50:15.835] [jointLog] [info] iteration = 1034 | max rel diff. = 0.00984625 [2019-03-14 07:50:15.835] [jointLog] [info] Finished optimizer [2019-03-14 07:50:15.835] [jointLog] [info] writing output [2019-03-14 07:50:15.846] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: female_bleached_K5_04/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl female_bleached_K5_04/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > female_bleached_K5_04/quant.sf.genes CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P09-GTGAAA-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P09-GTGAAA-READ2-Sequences.txt.gz_val_2.fq.gz) -o female_bleached_K5_05 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { female_bleached_K5_05 } ### [ threads ] => { 23 } Logs will be written to female_bleached_K5_05/logs [2019-03-14 07:50:16.139] [jointLog] [info] parsing read library format [2019-03-14 07:50:16.139] [jointLog] [info] There is 1 library. [2019-03-14 07:50:16.206] [stderrLog] [info] Loading Suffix Array [2019-03-14 07:50:16.206] [jointLog] [info] Loading Quasi index [2019-03-14 07:50:16.206] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 07:50:16.248] [stderrLog] [info] Loading Transcript Info [2019-03-14 07:50:16.258] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 07:50:16.262] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 07:50:16.264] [stderrLog] [info] Computing transcript lengths [2019-03-14 07:50:16.264] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 07:50:16.624] [stderrLog] [info] Done loading index [2019-03-14 07:50:16.624] [jointLog] [info] done [2019-03-14 07:50:16.624] [jointLog] [info] Index contained 5838 targets  processed 500000 fragments hits: 88719, hits per frag: 0.214713 processed 1000000 fragments hits: 177954, hits per frag: 0.178668 processed 1500001 fragments hits: 265464, hits per frag: 0.18064 processed 2000000 fragments hits: 354136, hits per frag: 0.185483 processed 2500000 fragments hits: 442324, hits per frag: 0.185368 processed 3000000 fragments hits: 531364, hits per frag: 0.181391 processed 3500000 fragments hits: 619408, hits per frag: 0.180571 processed 4000000 fragments hits: 706952, hits per frag: 0.178882 processed 4500000 fragments hits: 796458, hits per frag: 0.179743 processed 5000000 fragments hits: 884782, hits per frag: 0.180595 processed 5500000 fragments hits: 972779, hits per frag: 0.178674 processed 6000000 fragments hits: 1061327, hits per frag: 0.179253 processed 6500001 fragments hits: 1150077, hits per frag: 0.178657 processed 7000001 fragments hits: 1238239, hits per frag: 0.177441 processed 7500001 fragments hits: 1326006, hits per frag: 0.17775 processed 8000001 fragments hits: 1414141, hits per frag: 0.179339 processed 8500000 fragments hits: 1502621, hits per frag: 0.177795 processed 9000000 fragments hits: 1591912, hits per frag: 0.177641 processed 9500000 fragments hits: 1680350, hits per frag: 0.177089 processed 10000000 fragments hits: 1768790, hits per frag: 0.177108 processed 10500001 fragments hits: 1856601, hits per frag: 0.178497 processed 11000001 fragments hits: 1943744, hits per frag: 0.177185 processed 11500000 fragments hits: 2031731, hits per frag: 0.17764 processed 12000000 fragments hits: 2119210, hits per frag: 0.176803 processed 12500000 fragments hits: 2207321, hits per frag: 0.177293 processed 13000000 fragments hits: 2294894, hits per frag: 0.177514 processed 13500000 fragments hits: 2382921, hits per frag: 0.177565 processed 14000000 fragments hits: 2470709, hits per frag: 0.177661 processed 14500000 fragments hits: 2559344, hits per frag: 0.177099 processed 15000000 fragments hits: 2647984, hits per frag: 0.177649 processed 15500000 fragments hits: 2735970, hits per frag: 0.177426 processed 16000000 fragments hits: 2824293, hits per frag: 0.177522 processed 16500000 fragments hits: 2913049, hits per frag: 0.177249 processed 17000000 fragments hits: 3001330, hits per frag: 0.176912 processed 17500000 fragments hits: 3089627, hits per frag: 0.177478 processed 18000000 fragments hits: 3177806, hits per frag: 0.177349 processed 18500000 fragments hits: 3265062, hits per frag: 0.17713 processed 19000000 fragments hits: 3352245, hits per frag: 0.177403 processed 19500001 fragments hits: 3440154, hits per frag: 0.177778 processed 20000000 fragments hits: 3527540, hits per frag: 0.177031 processed 20500000 fragments hits: 3615081, hits per frag: 0.176638 processed 21000000 fragments hits: 3702982, hits per frag: 0.176692 [2019-03-14 07:51:08.957] [jointLog] [info] Computed 6922 rich equivalence classes for further processing [2019-03-14 07:51:08.957] [jointLog] [info] Counted 1916701 total reads in the equivalence classes [2019-03-14 07:51:08.967] [jointLog] [warning] 0.0194906% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 07:51:08.968] [jointLog] [warning] Only 1916701 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 07:51:08.968] [jointLog] [info] Mapping rate = 8.75913% [2019-03-14 07:51:08.968] [jointLog] [info] finished quantifyLibrary() [2019-03-14 07:51:08.968] [jointLog] [info] Starting optimizer [2019-03-14 07:51:08.974] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 07:51:08.974] [jointLog] [info] iteration = 0 | max rel diff. = 428.663 [2019-03-14 07:51:08.993] [jointLog] [info] iteration = 100 | max rel diff. = 0.112614 [2019-03-14 07:51:09.010] [jointLog] [info] iteration = 200 | max rel diff. = 0.0561104 [2019-03-14 07:51:09.025] [jointLog] [info] iteration = 300 | max rel diff. = 0.0366457 [2019-03-14 07:51:09.040] [jointLog] [info] iteration = 400 | max rel diff. = 0.0243093 [2019-03-14 07:51:09.056] [jointLog] [info] iteration = 500 | max rel diff. = 0.0243107 [2019-03-14 07:51:09.071] [jointLog] [info] iteration = 600 | max rel diff. = 0.0175284 [2019-03-14 07:51:09.086] [jointLog] [info] iteration = 700 | max rel diff. = 0.0150941 [2019-03-14 07:51:09.101] [jointLog] [info] iteration = 800 | max rel diff. = 0.0129989 [2019-03-14 07:51:09.116] [jointLog] [info] iteration = 900 | max rel diff. = 0.0117464 [2019-03-14 07:51:09.131] [jointLog] [info] iteration = 1000 | max rel diff. = 0.0117466 [2019-03-14 07:51:09.140] [jointLog] [info] iteration = 1050 | max rel diff. = 0.00947599 [2019-03-14 07:51:09.140] [jointLog] [info] Finished optimizer [2019-03-14 07:51:09.140] [jointLog] [info] writing output [2019-03-14 07:51:09.151] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: female_bleached_K5_05/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl female_bleached_K5_05/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > female_bleached_K5_05/quant.sf.genes CMD: salmon quant -i /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx -l ISR -1 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P13-ATCACG-READ1-Sequences.txt.gz_val_1.fq.gz) -2 <(gunzip -c /mnt/data/coral_RNAseq_2017/porites/20180311_fastqc_trimming/trimmed/4R041-L7-P13-ATCACG-READ2-Sequences.txt.gz_val_2.fq.gz) -o female_bleached_K5_06 -p 23 Version Info: ### PLEASE UPGRADE SALMON ### ### A newer version of Salmon with important bug fixes and improvements is available. #### ### The newest version, available at https://github.com/COMBINE-lab/salmon/releases contains new features, improvements, and bug fixes; please upgrade at your earliest convenience. ### ### salmon (mapping-based) v0.9.1 ### [ program ] => salmon ### [ command ] => quant ### [ index ] => { /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.salmon_quasi.idx } ### [ libType ] => { ISR } ### [ mates1 ] => { /dev/fd/63 } ### [ mates2 ] => { /dev/fd/62 } ### [ output ] => { female_bleached_K5_06 } ### [ threads ] => { 23 } Logs will be written to female_bleached_K5_06/logs [2019-03-14 07:51:09.666] [jointLog] [info] parsing read library format [2019-03-14 07:51:09.666] [jointLog] [info] There is 1 library. [2019-03-14 07:51:09.734] [stderrLog] [info] Loading Suffix Array [2019-03-14 07:51:09.733] [jointLog] [info] Loading Quasi index [2019-03-14 07:51:09.734] [jointLog] [info] Loading 32-bit quasi index [2019-03-14 07:51:09.772] [stderrLog] [info] Loading Transcript Info [2019-03-14 07:51:09.782] [stderrLog] [info] Loading Rank-Select Bit Array [2019-03-14 07:51:09.786] [stderrLog] [info] There were 5838 set bits in the bit array [2019-03-14 07:51:09.788] [stderrLog] [info] Computing transcript lengths [2019-03-14 07:51:09.788] [stderrLog] [info] Waiting to finish loading hash [2019-03-14 07:51:10.123] [jointLog] [info] done [2019-03-14 07:51:10.123] [jointLog] [info] Index contained 5838 targets [2019-03-14 07:51:10.123] [stderrLog] [info] Done loading index  processed 500000 fragments hits: 74582, hits per frag: 0.178033 processed 1000000 fragments hits: 149583, hits per frag: 0.164965 processed 1500000 fragments hits: 224829, hits per frag: 0.153829 processed 2000000 fragments hits: 298950, hits per frag: 0.153434 processed 2500000 fragments hits: 373792, hits per frag: 0.153695 processed 3000001 fragments hits: 447987, hits per frag: 0.156108 processed 3500000 fragments hits: 522449, hits per frag: 0.149913 processed 4000000 fragments hits: 595311, hits per frag: 0.150412 processed 4500000 fragments hits: 669143, hits per frag: 0.150934 processed 5000000 fragments hits: 744482, hits per frag: 0.150365 processed 5500000 fragments hits: 819714, hits per frag: 0.149929 processed 6000000 fragments hits: 894795, hits per frag: 0.150399 processed 6500000 fragments hits: 968481, hits per frag: 0.150729 processed 7000000 fragments hits: 1042037, hits per frag: 0.149763 processed 7500000 fragments hits: 1116191, hits per frag: 0.150785 processed 8000000 fragments hits: 1189915, hits per frag: 0.150577 processed 8500000 fragments hits: 1262826, hits per frag: 0.149822 processed 9000000 fragments hits: 1338093, hits per frag: 0.150509 processed 9500000 fragments hits: 1413030, hits per frag: 0.149153 processed 10000000 fragments hits: 1487514, hits per frag: 0.149873 processed 10500000 fragments hits: 1561583, hits per frag: 0.149852 processed 11000000 fragments hits: 1636034, hits per frag: 0.149645 processed 11500000 fragments hits: 1710202, hits per frag: 0.149791 processed 12000000 fragments hits: 1783825, hits per frag: 0.148906 processed 12500000 fragments hits: 1857716, hits per frag: 0.149553 processed 13000000 fragments hits: 1930674, hits per frag: 0.148927 processed 13500000 fragments hits: 2006530, hits per frag: 0.148946 processed 14000000 fragments hits: 2081184, hits per frag: 0.1498 processed 14500000 fragments hits: 2156397, hits per frag: 0.149716 processed 15000000 fragments hits: 2230565, hits per frag: 0.149133 processed 15500000 fragments hits: 2304290, hits per frag: 0.149534 processed 16000000 fragments hits: 2377880, hits per frag: 0.149361 processed 16500000 fragments hits: 2451283, hits per frag: 0.149351 processed 17000000 fragments hits: 2524226, hits per frag: 0.1489 [2019-03-14 07:51:49.754] [jointLog] [info] Computed 6627 rich equivalence classes for further processing [2019-03-14 07:51:49.754] [jointLog] [info] Counted 1294477 total reads in the equivalence classes [2019-03-14 07:51:49.764] [jointLog] [warning] 0.0100547% of fragments were shorter than the k used to build the index (31). If this fraction is too large, consider re-building the index with a smaller k. The minimum read size found was 20. [2019-03-14 07:51:49.764] [jointLog] [warning] Only 1294477 fragments were mapped, but the number of burn-in fragments was set to 5000000. The effective lengths have been computed using the observed mappings. [2019-03-14 07:51:49.764] [jointLog] [info] Mapping rate = 7.48881% [2019-03-14 07:51:49.764] [jointLog] [info] finished quantifyLibrary() [2019-03-14 07:51:49.765] [jointLog] [info] Starting optimizer [2019-03-14 07:51:49.769] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate [2019-03-14 07:51:49.770] [jointLog] [info] iteration = 0 | max rel diff. = 305.918 [2019-03-14 07:51:49.787] [jointLog] [info] iteration = 100 | max rel diff. = 0.113947 [2019-03-14 07:51:49.802] [jointLog] [info] iteration = 200 | max rel diff. = 0.0606269 [2019-03-14 07:51:49.816] [jointLog] [info] iteration = 300 | max rel diff. = 0.0432703 [2019-03-14 07:51:49.831] [jointLog] [info] iteration = 400 | max rel diff. = 0.0293281 [2019-03-14 07:51:49.846] [jointLog] [info] iteration = 500 | max rel diff. = 0.0218206 [2019-03-14 07:51:49.861] [jointLog] [info] iteration = 600 | max rel diff. = 0.0174288 [2019-03-14 07:51:49.876] [jointLog] [info] iteration = 700 | max rel diff. = 0.0147404 [2019-03-14 07:51:49.891] [jointLog] [info] iteration = 800 | max rel diff. = 0.0118515 [2019-03-14 07:51:49.908] [jointLog] [info] iteration = 900 | max rel diff. = 0.0111468 [2019-03-14 07:51:49.926] [jointLog] [info] iteration = 1000 | max rel diff. = 0.0113525 [2019-03-14 07:51:49.942] [jointLog] [info] iteration = 1091 | max rel diff. = 0.00876308 [2019-03-14 07:51:49.942] [jointLog] [info] Finished optimizer [2019-03-14 07:51:49.942] [jointLog] [info] writing output [2019-03-14 07:51:49.953] [jointLog] [warning] NOTE: Read Lib [( /dev/fd/63, /dev/fd/62 )] : Greater than 5% of the fragments disagreed with the provided library type; check the file: female_bleached_K5_06/lib_format_counts.json for details CMD: /home/shared/Trinityrnaseq-v2.6.6/util/support_scripts/salmon_trans_to_gene_results.pl female_bleached_K5_06/quant.sf /media/sam/4TB_toshiba/porites/20190129_sp_coral_transcripts/Trinity.sp.coral.fasta.gene_trans_map > female_bleached_K5_06/quant.sf.genes