FastQCFastQC Report
Mon 4 Jan 2021
C29_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC29_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17900262
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1421500.7941224547439585TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT203870.11389218772328584TruSeq Adapter, Index 7 (97% over 35bp)
GGCAATTTTATTATTTTTTTGTGTGTGATTTTTCAGTTCTTTTATATTTT188120.1050934338279518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)