FastQCFastQC Report
Fri 4 Oct 2024
68--1D9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename68--1D9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22944943
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCAGGGATCTGGGGG838570.36547050912264195TruSeq Adapter, Index 10 (97% over 37bp)
AGGACGAGGATGGAGATTATGAAGAGCTGATGCTCGCCCTCCCGTCCCAG278990.12159106257095519No Hit
GGCTAGATGAGCAGAGAAGACCCCCGAAGAGCATCACCCCAACCCCAAAG262990.11461784847319081No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255980.11156270904660778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)