FastQCFastQC Report
Fri 4 Oct 2024
109--2E2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename109--2E2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17143803
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2692341.5704450173628335No Hit
GGAAAACTAAGATGGATTAAACTGGACGGGAAGTTCGAAAGAGCGAAGAA754250.439954892155492No Hit
GGGAAAACTAAGATGGATTAAACTGGACGGGAAGTTCGAAAGAGCGAAGA408220.2381151953274311No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382810.2232935131137473No Hit
AGGACGAGGATGGAGATTATGAAGAGCTGATGCTCGCCCTCCCGTCCCAG375210.21886042437608502No Hit
GGCTAGATGAGCAGAGAAGACCCCCGAAGAGCATCACCCCAACCCCAAAG356420.20790019577336488No Hit
CCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAA236040.13768240337339388No Hit
TTTGTGAGCTTTCAGGGTGAGTCCATTTTCCGAGCTGTTGAAAATTGATG199210.11619942203022282No Hit
CTAAGATGGATTAAACTGGACGGGAAGTTCGAAAGAGCGAAGAAGAGGCT188930.11020308621138494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)