FastQCFastQC Report
Fri 4 Oct 2024
109--2E2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename109--2E2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17143803
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCAAAATCTGGGGG1746851.0189396133401674TruSeq Adapter, Index 1 (97% over 36bp)
GGCTAGATGAGCAGAGAAGACCCCCGAAGAGCATCACCCCAACCCCAAAG384420.22423262796475207No Hit
AGGACGAGGATGGAGATTATGAAGAGCTGATGCTCGCCCTCCCGTCCCAG369340.21543644662739067No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCAAAATCTAGGGG303340.17693857074769231TruSeq Adapter, Index 1 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222690.12989533302500034No Hit
GGTTCCTTGACATGCTCTAGTTAAACTGGTTTGAGTAAAGTCACTTTTTT211880.12358984759682551No Hit
CTGGTGCTTCTAGTGGGTACTGAACTTCAGCACGTCATCGCTCAGTTGGC200590.11700437761679834No Hit
CGGAATCTCGTTTGATTTCTCTGCCTCCCTCTGATACTAAGATGATTCAT188120.1097306122801341No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)