FastQCFastQC Report
Fri 4 Oct 2024
108--2D2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename108--2D2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23636244
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACCTGGATCTGGGGG1355350.5734202100807556TruSeq Adapter, Index 18 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529630.22407536493530866No Hit
GGCTAGATGAGCAGAGAAGACCCCCGAAGAGCATCACCCCAACCCCAAAG286950.12140253756053626No Hit
AGGACGAGGATGGAGATTATGAAGAGCTGATGCTCGCCCTCCCGTCCCAG282140.11936752726025336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)