FastQCFastQC Report
Fri 16 Feb 2024
sRNA-POC-57-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-57-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14136486
Total Bases341 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG9790286.9255400528816No Hit
TGAAAATCTTTGCTCTGAAGTGGAA7137595.049055331006588No Hit
CTCGGGCTGAGACTTGAAGCA4736473.3505285542673056No Hit
GTTGGATCCGGCCCGGACTGGCC2480941.7549905966730346No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2083751.4740226107110352No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1850041.3086986398175615No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1713921.2124087980563203No Hit
CGACTGCTGCCGGTCGTTGGGGCG1668611.1803569854630067No Hit
CAGCTGTGACCCTCGGGT1506561.0657245372011122No Hit
CTCGGGCTGAGACTTGAAGC1387040.9811773590692907No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA1278190.9041780255715601No Hit
CTCGGGCTGAGACTTGAAGCGGTT1176860.8324982601758315No Hit
CGACTGCTGCCGGTCGTTGGGGCA970700.6866628665709428No Hit
GTTGGATCCGGCCCGGACTGGCA954060.6748919073665124No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA773320.5470383516808915No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT704240.4981718936374995No Hit
CGTGGTGGACTGCTTGCAGTGCT665480.4707534814521798No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC641540.453818579808306No Hit
CTCGGGCTGAGACTTGAAGCGG639940.45268675680787995No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA608320.43031910476196134No Hit
GTTGGATCCGGCCCGGACTGGCT594990.42088960438966233No Hit
CACTTTCTGGAGTTAAGGCT585260.41400670576832177No Hit
GCACTGTGGTTCAGTGGTAGAATTCT570090.4032756089455329No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC543970.38479859846357856No Hit
GGAAGGAATCGGCCGTGGATTGGCC526560.3724829494401933No Hit
CGGACTGGCCGTGGTCGAGGC494110.3495281642128037No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC489690.3464015031741268No Hit
TTCCGAGGTCGCGGTCGGACCG480750.3400774421592466No Hit
CTTTCCGAGGTCGCGGTC479090.3389031757963047No Hit
CGGACTGGCCGTGGTCGAGGT472160.3340009674257096No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT453410.3207374166394676No Hit
CAGCTGTGACCCTCGGGTC435090.30777804328458996No Hit
CGACTGCTGCCGGTCGTTGGGGC405250.28666954432664526No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT398420.2818380748935768No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC398060.281583414718481No Hit
TCAAGTCTAGGCTGGTTAGTTT364320.2577160971969979No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG362180.25620228393392813No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA354470.2507483118506254No Hit
CAGCTGTGACCCTCGGGC344060.2433843884541038No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC338420.2393947123776022No Hit
AGACTTGAAGCGGTTGGAT335330.23720887920802952No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC331860.23475423807585563No Hit
GTTGGATCCGGCCCGGACT324600.2296185912114227No Hit
TCACTTTCTGGAGTTAAGGCT321950.22774400936696715No Hit
TTCCGAGGTCGCGGTCGGACCA318110.2250276341659448No Hit
TCTCGGGCTGAGACTTGAAGCG310680.21977173110771658No Hit
GTTGGATCCGGCCCGGACTGGC307760.21770615413193914No Hit
GACTGCTGCCGGTCGTTGGGGCG277530.1963217733176406No Hit
GAGGTGTAGAATAAGTGGGAGCAGCA265260.18764210568312378No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGA259120.18329873491898907No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC255260.18056821193046135No Hit
CTCGGGCTGAGACTTGAAGCGGTTG250490.17719396461044137No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCG243270.1720866133210191No Hit
CTCGGGCTGAGACTTGAAGCT239310.16928535139496478No Hit
CACTTTCTGGAGTTAAGGCA233310.1650410151433673No Hit
CGTGGTGGACTGCTTGCAGTGCA230310.1629188470175686No Hit
ACCCGTAGATCCGAACTTGT229330.16222560542980766No Hit
GGAAGGAATCGGCCGTGGATTGGCA229210.1621407187047757No Hit
CGAGTGTGCTCTTAACTG228870.16190020631718519No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC221720.15684237228403153No Hit
CTCGGGCTGAGACTTGAAGCGGTC219180.1550456032708553No Hit
CTCGGGCTGAGACTTGAAGCGGT215360.15234337585733823No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG211970.14994532587518566No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTCA211510.14961992676256322No Hit
TGAAAATCTTTGCTCTGAAGTGGAT207810.1470025860740781No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA207080.14648619183013373No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC206330.14595564979868406No Hit
CTCGGGCTGAGACTTGAAGCGA204630.14475308786073143No Hit
CTTTCTGGAGTTAAGGCT203450.14391836839791727No Hit
GACCAAGATAGTCGAACTCGACCCTGTC203440.1439112945041646No Hit
GACCAAGATAGTCGAACTCGAC200040.14150617062825938No Hit
TCGAACTCGACCCTGTCA197270.1395467020587719No Hit
CGGACTGGCCGTGGTCGAGG197160.13946888922749262No Hit
GACTTGAAGCGGTTGGAT192680.13629978482629984No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGC190940.13506892731333658No Hit
CTCGGGCTGAGACTTGAAGT190430.1347081587319508No Hit
TTCCGAGGTCGCGGTCGGACC187000.13228181317478757No Hit
GAATCGGCCGTGGATTGGC186740.13209789193721835No Hit
CGGACTGGCCGTGGTCGAGGTGA183060.12949469903623856No Hit
ACCCGTAGATCCGAACTTGTGG178650.12637511189131442No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCA177740.12573138755982213No Hit
GCCGAGCTGTTCTTCCTCGCCGAGAC177170.1253281756159204No Hit
GAGGTGTAGAATAAGTGGGAGCAGCC173750.12290890395250983No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCA172880.12229347519602819No Hit
GGAAGGAATCGGCCGTGGATTGGCCCA166310.11764592700052898No Hit
GCACTGGTGGTTCAGTGGTAGAATTCA163990.1160047836499113No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCG163700.11579964073108409No Hit
GACTGCTGCCGGTCGTTGGGGCA163190.11543887214969832No Hit
TGAAAATCTTTGCTCTGAAGTGGA161480.11422923631799302No Hit
GAGACTTGAAGCGGTTGGAT161330.11412312791170308No Hit
AGACTTGAAGCGGTTGGATCCGGCCC160430.11348647747396348No Hit
CGTGGTGGACTGCTTGCAGTGCC160050.1132176695113623No Hit
TCTCGGGCTGAGACTTGAAGCA154080.10899455494102282No Hit
CACTTTCTGGAGTTAAGGCC148530.10506854390829516No Hit
CTCGGGCTGAGACTTGAAGCGGTTGG147150.10409234657042775No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCA146710.10378109524531061No Hit
CCTTTCCGAGGTCGCGGTC144540.10224606030098286No Hit
GACTTGAAGCGGTTGGATCCGGCCC141590.10015926164394745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)