FastQCFastQC Report
Fri 16 Feb 2024
sRNA-POC-53-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-53-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15732665
Total Bases392.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG166264710.568120531391218No Hit
TGAAAATCTTTGCTCTGAAGTGGAA9547626.068660331863674No Hit
TGAAAATCTTTGCTCTGAGGTGGAA5599553.559187206998941No Hit
CTCGGGCTGAGACTTGAAGCA4932093.134936134469271No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1696731.0784758971223247No Hit
GTTGGATCCGGCCCGGACTGGCC1364970.8676025326923316No Hit
CTCGGGCTGAGACTTGAAGC1051610.668424580323804No Hit
CTCGGGCTGAGACTTGAAGCGGTT1045800.6647316268413521No Hit
CGGACTGGCCGTGGTCGAGGC1031770.6558138751444844No Hit
TCTCGGGCTGAGACTTGAAGCG1028970.6540341385264353No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1006180.6395483536959568No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC901530.5730306975963704No Hit
TGAAAATCTTTGCTCTGAAGTGGAG842680.5356244476063019No Hit
CGACTGCTGCCGGTCGTTGGGGCG809350.5144392256493099No Hit
CTCGGGCTGAGACTTGAAGCGG765640.4866562658011215No Hit
ACCCGTAGATCCGAACTTGTGG732370.4655091810573733No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG649800.4130260194315458No Hit
TCAAGTCTAGGCTGGTTAGTTT584850.37174248609501315No Hit
CTCGGGCTGAGACTTGAAACG566880.36032039072846206No Hit
TGAAAATCTTTGCTCTGAGGTGGAG548820.3488410895420452No Hit
GTTGGATCCGGCCCGGACTGGCA534130.3395038284994945No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA525390.33394850777029833No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC509150.3236260353856133No Hit
TCTTGCATCACTTTTAAATAATTCTGTGGA503490.32002842493627115No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA497800.316411745880307No Hit
ACCCGTAGATCCGAACTTGT458940.2917115441026679No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA434390.2761070676837014No Hit
CTTTCCGAGGTCGCGGTC408720.2597906966175152No Hit
GGAAGGAATCGGCCGTGGATTGGCC397370.2525764071122089No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCG378260.24042970469402355No Hit
CAGCTGTGACCCTCGGGT354370.22524473762074002No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG350560.22282302457975176No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC315830.20074793431373514No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC310270.19721388588646616No Hit
TCTCGGGCTGAGACTTGAAGCA308080.1958218776030634No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA303820.1931141354627458No Hit
GCACTGTGGTTCAGTGGTAGAATTCT300010.19069242242175755No Hit
CGACTGCTGCCGGTCGTTGGGGCA299340.1902665568738672No Hit
GTTGGATCCGGCCCGGACTGGCT290210.18446334425858557No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT282810.17975975462517No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG278120.17677869578993768No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC277420.1763337616354254No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC274500.17447775059088846No Hit
TGAAAATCTTTGCTCTGAAGTGG273680.17395654200988833No Hit
CTCGGGCTGAGACTTGAAGCGGTTG271410.1725136841088271No Hit
TGAAAATCTTTGCTCTGAGGTGG268790.17084835913050966No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC256120.16279505093383734No Hit
CCCTTTCCGAGGTCGCGGTCGGACCG237820.15116320089444477No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT229850.14609730773521207No Hit
GTTGGATCCGGCCCGGACTGGC226000.1436501698853945No Hit
GGATCCGGCCCGGACTGGC221570.14083437230755247No Hit
CAGCTGTGACCCTCGGGTC219230.13934702099104No Hit
TGAAAATCTTTGCTCTGAAGTGGAT216760.13777703904583233No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA214890.1365884292330638No Hit
CCCTTTCCGAGGTCGCGGTC213680.1358193287659783No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT212430.1350248034900635No Hit
CTCGGGCTGAGACTTGAAGCGGTC212320.134954885265783No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA208230.1323551985629898No Hit
GGATCCGGCCCGGACTGGCC204030.12968559363591609No Hit
TGAAAATCTTTGCTCTGAAGTGGA201210.12789314461345233No Hit
TGAAAATCTTTGCTCTGAGGTGGA198910.1264312181057691No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC198200.1259799277490495No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCG196440.1248612361605615No Hit
TCCCTTTCCGAGGTCGCGGTC195760.12442901441046383No Hit
GGACTGGCCGTGGTCGAGGC187070.1189054746923042No Hit
CGGACTGGCCGTGGTCGAGGT186040.11825078586495041No Hit
TTGTGTAACTCCCTAAGGAAGG185030.11760880944201126No Hit
CTTTCCGAGGTCGCGGTCGGACCG177240.11265732792251028No Hit
GCACTGGTGGTTCAGTGGTAGAATTCG177130.11258740969822977No Hit
CTCGGGCTGAGACTTGAAGCT172180.1094410896056072No Hit
TCTCGGGCTGAGACTTGAAGCGGTT171280.10886903140694852No Hit
TGATCATGGATTCTAAACTGGTAATGGCC171110.10876097596942413No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCA170910.10863385192527776No Hit
GGAAGGAATCGGCCGTGGATTGGCA166050.10554473765252105No Hit
GTTGGATCCGGCCCGGACTGGCG164560.10459766352363062No Hit
CGTGGTGGACTGCTTGCAGTGCT160030.10171830392371539No Hit
CTCGGGCTGAGACTTGAAGCGA158630.10082843561469083No Hit
GTTGGATCCGGCCCGGACT158380.10066953055950786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)