FastQCFastQC Report
Fri 16 Feb 2024
sRNA-POC-50-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-50-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12141955
Total Bases314.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA313595525.827430590872723No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3426092.8216955177317No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1259361.0371970576402234No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1255771.0342403673872946No Hit
CTCGGGCTGAGACTTGAAGCG1200520.9887369867537805No Hit
TCAAGTCTAGGCTGGTTAGTTT996790.820946873876571No Hit
CTCGGGCTGAGACTTGAAGCA880150.7248832663273748No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC871430.7177015562979767No Hit
ACCCGTAGATCCGAACTTGT758680.6248417161816199No Hit
TGAAAATCTTTGCTCTGAAGTGGA656630.5407942954820703No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT641830.5286051546064864No Hit
TGAAAATCTTTGCTCTGAAGTGGAT625750.5153618177632844No Hit
ACCCGTAGATCCGAACTTGTGG590630.48643731590176376No Hit
CGGACTGGCCGTGGTCGAGGC540590.44522484229269504No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC494900.40759498779232833No Hit
CTCGGGCTGAGACTTGAAGCGGTT447650.36868033195642713No Hit
TGAAAATCTTTGCTCTGAAGTGGAG447590.368630916520445No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT423000.34837882367378237No Hit
GTTGGATCCGGCCCGGACTGGCC384630.31677765236323147No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA376880.31039482521554396No Hit
TGAAAATCTTTGCTCTGAAGTGG371740.30616156953307766No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA348020.2866260005081554No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC344210.2834881203232923No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA310030.25533779362549114No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG297410.24494408025725675No Hit
TGAAAATCTTTGCTCTGAAGTGGGA277520.22856286322919167No Hit
ACCCGTAGATCCGAACTTGTGA227200.1871197842522065No Hit
TGAAAATCTTTACTCTGAAGTGGAA225180.18545613124080926No Hit
GTTGGATCCGGCCCGGACTGGCA216670.17844737523734852No Hit
CGAAAATCTTTGCTCTGAAGTGGAA204380.1683254467670157No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA187140.15412674482815988No Hit
ACCCGTAGATCCGAACTTGC180460.14862515962215309No Hit
TCAAGTCTAGGCTGGTTAGTTC179040.14745566097057683No Hit
TATTAATGCTGTAGGTGAAAGACA169840.13987862745332197No Hit
TGAAAATCTTTGCTCTGAAGTGA168320.1386267697417755No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT167900.13828086168990084No Hit
CACCCCTCATCCGCTACTTTGACCTCATT164870.13578538217280495No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT160860.1324827838680015No Hit
TCTGTTTCCATATTTGAACGCTGACGAC160680.1323345375600552No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA149120.12281383022750454No Hit
TGAAAATCTTTGCTCTGAGGTGGAA148340.1221714295597373No Hit
CTTTCCGAGGTCGCGGTC148200.12205612687577908No Hit
TCCCTTTCCGAGGTCGCGGTC143670.11832526145913076No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA136580.11248600410724632No Hit
TGAAAATCCTTGCTCTGAAGTGGAA136120.11210715243138358No Hit
TTGTGTAACTCCCTAAGGAAGG129780.10688558802927535No Hit
CAGCTGTGACCCTCGGGTC129730.10684440849929026No Hit
TTCCTGCACGGCTTCAGGCAATTCTGA126100.10385477462237341No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG123800.1019605162430597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)