FastQCFastQC Report
Fri 16 Feb 2024
sRNA-POC-48-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-48-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14045042
Total Bases353.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA323040723.000337058443826No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC10174677.244314399344623No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC2433431.7325900485025252No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1892221.3472512221750566No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1701131.2111960932548298No Hit
CTCGGGCTGAGACTTGAAGCG1443961.0280923332233538No Hit
CCCTTTCCGAGGTCGCGGTC1219390.8681996109374398No Hit
TCCCTTTCCGAGGTCGCGGTC1204520.8576122449473629No Hit
CTTTCCGAGGTCGCGGTC1170960.8337176919798459No Hit
CTCCCTTTCCGAGGTCGCGGTC984570.7010089396671081No Hit
CTCGGGCTGAGACTTGAAGCA975010.694202267248471No Hit
GGATCCGGCCCGGACTGGCC893360.6360678736311361No Hit
CTCGGGCTGAGACTTGAAGCGGTT837190.5960751131965287No Hit
GTTGGATCCGGCCCGGACTGGCC801230.5704717721741238No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA763450.5435726002100955No Hit
TCAAGTCTAGGCTGGTTAGTTT722730.5145801628788295No Hit
TGAAAATCTTTGCTCTGAAGTGGA706980.5033662412686271No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA680130.4842491749045678No Hit
TGAAAATCTTTGCTCTGAAGTGGAT588170.41877411260144326No Hit
ACCCGTAGATCCGAACTTGT582450.41470150107062687No Hit
CCTTTCCGAGGTCGCGGTC558360.3975495409696888No Hit
GGATCCGGCCCGGACTGGC532960.37946486738879104No Hit
TCTCGGGCTGAGACTTGAAGCGGTT509860.36301778236049415No Hit
TCTCGGGCTGAGACTTGAAGCG502870.35804093715063295No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC462850.32954689633537587No Hit
ACCCGTAGATCCGAACTTGTGG449360.3199420834768597No Hit
GGATCCGGCCCGGACTGGCA434530.3093831972876977No Hit
GTTGGATCCGGCCCGGACTGGCA406640.2895256560998536No Hit
TTCTGCTGAACTACTTTGAGAACGAGT384780.2739614448999156No Hit
GCACTGTGGTTCAGTGGTAGAATTCT381330.2715050620710141No Hit
TGAAAATCTTTGCTCTGAAGTGGAG372090.26492622805969535No Hit
TGAAAATCTTTGCTCTGAAGTGG342650.2439650945864028No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT339900.24200710827351032No Hit
TCTCGGGCTGAGACTTGAAGCA339210.24151583170773003No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTC317580.2261153793630521No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC307720.219095108437554No Hit
TGAAAATCTTTGCTCTGAAGTGGGA272380.19393320432932845No Hit
TGAAAATCTTTGCTCTGAGGTGGAA265410.1889705990199246No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC262870.18716213166183482No Hit
CTCGGGCTGAGACTTGAAGC254100.180917935311265No Hit
CGGACTGGCCGTGGTCGAGGC231860.1650831660026364No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG229230.1632106190924883No Hit
TCCCTTTCCGAGGTCGCGGTA225390.16047655820466752No Hit
CCCTTTCCGAGGTCGCGGTA222880.15868945069726384No Hit
CTTTCCGAGGTCGCGGTA221750.15788489632142075No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTC202480.144164752230716No Hit
GGATCCGGCCCGGACTGGCT201980.143808754719281No Hit
CGAAAATCTTTGCTCTGAAGTGGAA201490.14345987715807473No Hit
GTTGGATCCGGCCCGGACT198780.14153037064609703No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA197590.14068309656888175No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA197370.14052645766385036No Hit
CTCCCTTTCCGAGGTCGCGGTA189710.13507257578866622No Hit
TGGATCCGGCCCGGACTGGCC188640.13431074111419533No Hit
GACCAAGATAGTCGAACTCGAC188020.13386930420001592No Hit
CAGCTGTGACCCTCGGGTC185000.13171907923094853No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC183510.13065820664687225No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA182340.12982517247011435No Hit
GCACTGGTGGTTCAGTGGTAGAATTCA176550.1257027212876971No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG175780.12515448512008723No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC175780.12515448512008723No Hit
GTTGGATCCGGCCCGGACTGGCT173870.12379457462640554No Hit
ACCCGTAGATCCGAACTTGTGA171020.12176538881122606No Hit
CTCGGGCTGAGACTTGAAGCGGT164760.11730829996805991No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTA158380.11276577172214935No Hit
CTCGGGCTGAGACTTGAAGCGGTC154620.11008867043615818No Hit
CACTACCGATAGCCAACTCGAACGTTTCCA153200.1090776375036828No Hit
TGAAAATCCTTGCTCTGAAGTGGAA152810.1087999594447635No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGT148280.10557462199116242No Hit
TTGTGTAACTCCCTAAGGAAGG145610.10367359528009955No Hit
TCTCCCTTTCCGAGGTCGCGGTC145480.10358103592712645No Hit
CTCGGGCTGAGACTTGAAGCGG142770.10165152941514878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)