FastQCFastQC Report
Fri 16 Feb 2024
sRNA-POC-47-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-47-S1-TP2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12066776
Total Bases313.5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA222278018.42066182383762No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2953652.4477540645488074No Hit
TGAAGATCTTTGCTCTGAAGTGGAA1399881.1601110354580213No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1224701.0149355552800516No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1031570.8548845192783889No Hit
ACCCGTAGATCCGAACTTGT999600.8283902841985299No Hit
TCAAGTCTAGGCTGGTTAGTTT948150.7857525489824291No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA907710.752239040486042No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC905740.7506064585934139No Hit
CTCGGGCTGAGACTTGAAGCGGTT819210.6788971635837112No Hit
CCCTTTCCGAGGTCGCGGTC742670.6154667990853563No Hit
CTTTCCGAGGTCGCGGTC733250.6076602399845659No Hit
CTCCCTTTCCGAGGTCGCGGTC597960.49554247132788415No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT551690.4571975148954452No Hit
ACCCGTAGATCCGAACTTGTGG532440.441244620766972No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT500640.4148912683885074No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC499190.41368962181779123No Hit
TGAAAATCTTTGCTCTGAAGTGGAT493230.4087504400512614No Hit
CTCGGGCTGAGACTTGAAGCG460870.38193300347996845No Hit
TGAAAATCTTTGCTCTGAAGTGGA452770.37522035711941615No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC388220.3217263666782246No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG371950.3082430634330164No Hit
CTCGGGCTGAGACTTGAAGCA368900.3057154620256479No Hit
GGATCCGGCCCGGACTGGC345730.2865139785473767No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA336690.27902233372029117No Hit
TGAAAATCTTTGCTCTGAAGTGGAG316450.26224900503663945No Hit
ACCCGTAGATCCGAACTTGC280690.23261391443746035No Hit
TGAAAATCTTTGCTCTGAGGTGGAA259820.2153184910368768No Hit
ACCCGTAGATCCGAACTTGTGA244700.20278821783051248No Hit
TCTCGGGCTGAGACTTGAAGCGGTT242280.20078271114007587No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA235090.19482420159286953No Hit
TGAAAATCTTTGCTCTGAAGTGG229470.1901667852291283No Hit
GGATCCGGCCCGGACTGGCC226370.18759774773311447No Hit
TTCCTGCACGGCTTCAGGCAATTCTGA224570.18610604854188062No Hit
CTCGGGCTGAGACTTGAAGCGGTC218280.18089338859029122No Hit
TTTCCATATTTGAACGCTGACGACAGTAC214940.17812545786877954No Hit
TCAAGTCTAGGCTGGTTAGTTC213300.17676635416121092No Hit
TGAAAATCTTTGCTCTGAAGTGGGA209240.1734017437632057No Hit
TCTGTTTCCATATTTGAACGCTGACGAC198280.16431895313213735No Hit
CAGCTGTGACCCTCGGGTC197890.16399575164070337No Hit
AACTGCTGAGATTCTATGGATTT194330.16104550212915197No Hit
TTTCCATATTTGAACGCTGACGACAGTACA193380.16025821644488966No Hit
TTTCCATATTGAACGCTGACGACAGTACGTC176600.14635226509549856No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT174320.14446277945326905No Hit
TTCTGCTGAACTACTTTGAGAACGAGT173830.14405670578454427No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA166720.13816449397917058No Hit
AACTTTTGACGGTGGATCT165630.1372611872467012No Hit
GCACTGTGGTTCAGTGGTAGAATTCT158030.13096290177260272No Hit
TCAGTCCCACCATCTCACCAAT156470.12967009580686673No Hit
TCCCTTTCCGAGGTCGCGGTC154150.1277474612937209No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA153080.12686072899670964No Hit
TTGTGTAACTCCCTAAGGAAGG151680.12570051851463887No Hit
CGAAAATCTTTGCTCTGAAGTGGAA149820.12415909601703055No Hit
ATCCATAGAACTTCTGCATTTGA148940.12342982085687179No Hit
TAGGAATTGCCCTGGACTGCAGTAGAGGC147250.1220292810606578No Hit
TTTCCATATTTGAACGCTGACGACAGTAGG141170.11699065268137901No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC139300.11544094296604163No Hit
CTCGGGCTGAGACTTGAAGCGGT134040.11108186644054718No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT133030.11024485744991039No Hit
TTCCAAATCTGTGATGTTATTGA131130.10867028608138579No Hit
CTCGGGCTGAGACTTGAAGC129750.10752665003477316No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC128890.10681394931007254No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA125400.1039217103226247No Hit
CCCTTTCCGAGGTCGCGGTA124400.103092988549717No Hit
TGGAAATCTTTGCTCTGAAGTGGAA123440.10229741564772561No Hit
CTTTCCGAGGTCGCGGTA121300.10052395105370315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)