FastQCFastQC Report
Thu 15 Feb 2024
sRNA-POC-57-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-57-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16502248
Total Bases378.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG9649055.847112466131887No Hit
TGAAAATCTTTGCTCTGAAGTGGAA7055504.275478104558846No Hit
CTCGGGCTGAGACTTGAAGCA4690982.8426308948938352No Hit
GTTGGATCCGGCCCGGACTGGCC2458581.4898455046851797No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2070101.2544351533197173No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1831241.109691237218105No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1693841.0264298536781171No Hit
TTCCGAGGTCGCGGTC1645260.9969914401965114No Hit
CGACTGCTGCCGGTCGTTGGGGCG1634660.9905680729074002No Hit
CAGCTGTGACCCTCGGGT1487810.901580196831365No Hit
CTCGGGCTGAGACTTGAAGC1404550.8511264647095353No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA1265580.7669136956371035No Hit
CTCGGGCTGAGACTTGAAGCGGTT1162020.7041586091785798No Hit
GAGTGTGCTCTTAACTG1116670.6766775047860146No Hit
GAGTGTGCTCTTAACT1045380.6334773298765114No Hit
CGACTGCTGCCGGTCGTTGGGGCA944870.5725704764587225No Hit
GTTGGATCCGGCCCGGACTGGCA944680.5724553406299554No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA766870.46470638424534644No Hit
AGTGTGCTCTTAACT707000.4284264786227913No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT697290.422542431794747No Hit
CGTGGTGGACTGCTTGCAGTGCT658470.3990183640434928No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC637560.3863473630986518No Hit
AGTGTGCTCTTAACTG631810.38286298933333207No Hit
CTCGGGCTGAGACTTGAAGCGG630500.3820691580928853No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA602070.36484120224105226No Hit
GTTGGATCCGGCCCGGACTGGCT588620.3566907975204348No Hit
CACTTTCTGGAGTTAAGGCT578430.35051588123024213No Hit
GCACTGTGGTTCAGTGGTAGAATTCT564120.341844335389942No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC536810.32529507494978865No Hit
GGAAGGAATCGGCCGTGGATTGGCC518270.31406024197430554No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC488510.29602633532110295No Hit
CGGACTGGCCGTGGTCGAGGC487200.29523250408065616No Hit
TTCCGAGGTCGCGGTCGGACCG475690.28825769676955526No Hit
CTTTCCGAGGTCGCGGTC472410.2862700887782077No Hit
CGGACTGGCCGTGGTCGAGGT466140.2824706064288938No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT447670.2712781919166407No Hit
CGACTGCTGCCGGTCGTTGGGGC403230.24434852754606526No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC397040.24059752344044277No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT394230.2388947251307822No Hit
GAGTGTGCTCTTAACTA390160.23642839448298195No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG359520.21786122714917389No Hit
TCAAGTCTAGGCTGGTTAGTTT359120.21761883593071682No Hit
CAGCTGTGACCCTCGGGTC353530.2142314186527799No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA346380.2098986756228606No Hit
CAGCTGTGACCCTCGGGC340240.20617797041954525No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC335550.20333593338313663No Hit
AGACTTGAAGCGGTTGGAT331400.20082112449164502No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC327670.19856082637953326No Hit
GTTGGATCCGGCCCGGACT321580.19487042007852506No Hit
TCACTTTCTGGAGTTAAGGCT318000.1927010186733347No Hit
TTCCGAGGTCGCGGTCGGACCA315780.1913557474108982No Hit
GTTGGATCCGGCCCGGACTGGC314630.19065887265783424No Hit
CTTTCCGAGGTCGCG312510.18937419920001203No Hit
TCTCGGGCTGAGACTTGAAGCG306040.18545352124146966No Hit
GAATCGGCCGTGGAT276500.16755292975841837No Hit
GACTGCTGCCGGTCGTTGGGGCG272650.1652199142807695No Hit
CTCGGGCTGAGACTTGA270280.16378374631141163No Hit
GAGGTGTAGAATAAGTGGGAGCAGCA263100.15943282394010805No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGA256060.1551667384952644No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC254420.1541729344995906No Hit
CTCGGGCTGAGACTTGAAGCGGTTG246740.14951902310521573No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCG240600.1457983179019004No Hit
TTCCGAGGTCGCGGTA240300.14561652448805762No Hit
CTCGGGCTGAGACTTGAAGCT236680.14342288396102157No Hit
AGCTGTGACCCTCGGGT236660.1434107644000987No Hit
CACTTTCTGGAGTTAAGGCA230770.139841553708319No Hit
GAATCGGCCGTGGATT229900.139314352808175No Hit
CGTGGTGGACTGCTTGCAGTGCA228690.1385811193723425No Hit
ACCCGTAGATCCGAACTTGT226450.13722372854898315No Hit
CGAGTGTGCTCTTAACTG224860.13626022345561647No Hit
AGTGTGCTCTTAACTGA224430.13599965289577517No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC221440.13418777853780892No Hit
AGTGTGCTCTTAACTA220340.1335212026870521No Hit
GGAAGGAATCGGCCGTGGATTGGCA218370.13232742593615124No Hit
CTCGGGCTGAGACTTGAAGCGGTC216790.13136998062324598No Hit
CTCGGGCTGAGACTTGAAGCGA216510.13120030677032607No Hit
CTCGGGCTGAGACTTGAAGCGGT215140.13037011684711078No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTCA210400.12749778090839503No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG209620.12702511803240382No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC205590.1245830265064493No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA205070.12426791792245516No Hit
CGAGTGTGCTCTTAACT203560.1233528910727799No Hit
GAGTGTGCTCTTAACC203510.12332259217047278No Hit
TGAAAATCTTTGCTCTGAAGTGGAT203140.12309838029340003No Hit
GACCAAGATAGTCGAACTCGACCCTGTC202010.12241362510125894No Hit
CTTTCTGGAGTTAAGGCT201060.12183794595742349No Hit
GACCAAGATAGTCGAACTCGAC198280.12015332698914717No Hit
CGGACTGGCCGTGGTCGAGG196570.11911710453024339No Hit
TCGAACTCGACCCTGTCA195860.11868686011748217No Hit
CTTGAAGCGGTTGGA195060.11820207768056812No Hit
GACTTGAAGCGGTTGGAT190150.11522672547400814No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGC188900.11446925291632995No Hit
TTCCGAGGTCGCGGTCGGACC188180.1140329487231073No Hit
CTCGGGCTGAGACTTGAAGT187910.1138693346506488No Hit
GAATCGGCCGTGGATTGGC182560.11062735210378609No Hit
CGGACTGGCCGTGGTCGAGGTGA181540.1100092544967207No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCA178890.1084034126744429No Hit
GCCGAGCTGTTCTTCCTCGCCGAGAC176560.10699148382693072No Hit
ACCCGTAGATCCGAACTTGTGG175750.10650064160955526No Hit
GAGGTGTAGAATAAGTGGGAGCAGCC172480.10451909339866908No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCA172470.10451303361820766No Hit
TGAAAATCTTTGCTCTGAAGTGGA165900.10053175785505102No Hit
CTTTCCGAGGTCGCA165770.1004529807090525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)