FastQCFastQC Report
Thu 15 Feb 2024
sRNA-POC-53-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-53-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16831731
Total Bases409.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG16403039.745301894380322No Hit
TGAAAATCTTTGCTCTGAAGTGGAA9423755.598800265997597No Hit
TGAAAATCTTTGCTCTGAGGTGGAA5531923.286601954368211No Hit
CTCGGGCTGAGACTTGAAGCA4884712.902084164724353No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1683871.0004140394116325No Hit
GTTGGATCCGGCCCGGACTGGCC1355630.8054014171210316No Hit
CTCGGGCTGAGACTTGAAGC1075900.6392093599879893No Hit
CTCGGGCTGAGACTTGAAGCGGTT1021390.6068240990781043No Hit
CGGACTGGCCGTGGTCGAGGC1018930.6053625738196505No Hit
TCTCGGGCTGAGACTTGAAGCG1014150.6025226995369638No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT996220.5918702003970953No Hit
CTTTCCGAGGTCGCG894190.5312525491287854No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC891720.5297850827107444No Hit
CGACTGCTGCCGGTCGTTGGGGCG769530.45719005371461796No Hit
CTCGGGCTGAGACTTGAAGCGG755910.44909819435683707No Hit
TGAAAATCTTTGCTCTGAAGTGGAG733560.4358197026794214No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG642690.3818323855104386No Hit
TCAAGTCTAGGCTGGTTAGTTT572320.3400244454952375No Hit
CTCGGGCTGAGACTTGAAACG558500.3318137629457125No Hit
ACCCGTAGATCCGAACTTGTGG548820.3260627204652926No Hit
GTTGGATCCGGCCCGGACTGGCA528630.3140675192587144No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA520690.30935023854646915No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC505480.30031373481432183No Hit
TCTTGCATCACTTTTAAATAATTCTGTGGA497590.2956261599000127No Hit
TGAAAATCTTTGCTCTGAGGTGGAG497420.29552516018703007No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA492390.2925367569146632No Hit
ACCCGTAGATCCGAACTTGT440700.26182690300837147No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA430080.25551739152675385No Hit
CTTTCCGAGGTCGCGGTC403890.2399574945678493No Hit
GGAAGGAATCGGCCGTGGATTGGCC394090.23413515817238287No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCG376780.2238510109269213No Hit
CAGCTGTGACCCTCGGGT350560.2082732904892551No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC314670.18695046873075621No Hit
GAGTGTGCTCTTAACT309570.1839204773412788No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC306700.18221536453974937No Hit
TCTCGGGCTGAGACTTGAAGCA305210.18133013176125498No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA297340.17665443916612023No Hit
GCACTGTGGTTCAGTGGTAGAATTCT296450.17612567596285847No Hit
GTTGGATCCGGCCCGGACTGGCT287580.1708558674090027No Hit
CTTTCCGAGGTCGCA285050.1693527540334384No Hit
CGACTGCTGCCGGTCGTTGGGGCA284010.16873487343636848No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT279660.16615046901593186No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC276700.16439188577811753No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG274890.1633165358928324No Hit
GAGTGTGCTCTTAACGG272610.1619619515069484No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC271270.16116583612226218No Hit
GTTGGATCCGGCCCGGA267430.1588844308407733No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG264650.15723278847552877No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC254490.15119657033492276No Hit
CCCTTTCCGAGGTCGCGGTCGGACCG237470.14108471671749032No Hit
TGAAAATCTTTGCTCTGAAGTGG235660.14000936683220522No Hit
TGAAAATCTTTGCTCTGAGGTGG229750.1364981415161637No Hit
GTTGGATCCGGCCCGGACTGGC229480.13633773020730902No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT227930.1354168504712914No Hit
GGATCCGGCCCGGACTGGC219670.13050945265225544No Hit
CTCGGGCTGAGACTTGA215610.12809734185984795No Hit
CCCTTTCCGAGGTCGCGGTC210930.12531687917303336No Hit
TGAAAATCTTTGCTCTGAAGTGGAT210700.12518023250252752No Hit
CTCGGGCTGAGACTTGAAGCGGTC210400.12500199771491122No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA209960.12474058669307393No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT208640.12395635362756213No Hit
TGAAAATCTTTGCTCTGAAGTGGA207610.12334441419007945No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA205610.12215618227263733No Hit
GGATCCGGCCCGGACTGGCC203220.12073624513129397No Hit
TGAAAATCTTTGCTCTGAGGTGGA202380.1202371877259683No Hit
GAGTGTGCTCTTAACTG202330.12020748192803224No Hit
CAGCTGTGACCCTCGGG201520.11972624800146818No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC196530.11676160936745009No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCG194390.11549020121578701No Hit
TCCCTTTCCGAGGTCGCGGTC193310.11484855598036826No Hit
ACCCGTAGATCCGAACTTGGGG189460.11256120953929219No Hit
GGACTGGCCGTGGTCGAGGC184540.10963815902238457No Hit
CGGACTGGCCGTGGTCGAGGT183910.1092638659683903No Hit
CAGCTGTGACCCTCGGGTC183480.10900839610614024No Hit
CTTTCCGAGGTCGCGGTCGGACCG176990.10515258353404056No Hit
GCACTGGTGGTTCAGTGGTAGAATTCG175270.10413070408504034No Hit
CTCGGGCTGAGACTTGAAGCT170860.10151065270708046No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCA170230.10113635965308619No Hit
TGATCATGGATTCTAAACTGGTAATGGCC169930.10095812486546987No Hit
CTCGGGCTGAGACTTGAAGCGA169860.10091653674835939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)