FastQCFastQC Report
Thu 15 Feb 2024
sRNA-POC-50-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-50-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12625701
Total Bases321.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA309418924.507066973944656No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3390222.685173678673366No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1244430.9856324017177343No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1238350.9808168275171414No Hit
CTCGGGCTGAGACTTGAAGCG1181810.9360351555925489No Hit
TCAAGTCTAGGCTGGTTAGTTT979960.7761628443442468No Hit
CTCGGGCTGAGACTTGAAGCA870380.689371623801324No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC862000.6827343685709015No Hit
ACCCGTAGATCCGAACTTGT747390.5919592108192646No Hit
TGAAAATCTTTGCTCTGAAGTGGA680280.5388057265097598No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT633550.5017939201950053No Hit
TGAAAATCTTTGCTCTGAAGTGGAT612000.4847255609807329No Hit
ACCCGTAGATCCGAACTTGTGG578280.4580181330129709No Hit
CGGACTGGCCGTGGTCGAGGC532050.42140234431339696No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC486870.3856181926057016No Hit
TGAAAATCTTTGCTCTGAAGTGGAG442840.35074488141292115No Hit
CTCGGGCTGAGACTTGAAGCGGTT441230.3494697046920405No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT421590.33391413276775683No Hit
GTTGGATCCGGCCCGGACTGGCC377940.29934179496251334No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA372740.2952232117646379No Hit
TGAAAATCTTTGCTCTGAAGTGG365210.28925918648002197No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA343970.2724363581871613No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC338780.26832569534158934No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA305350.241847957590632No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG294650.23337318062577275No Hit
TGAAAATCTTTGCTCTGAAGTGGGA274060.2170651752326465No Hit
ACCCGTAGATCCGAACTTGTGA224560.1778594313297931No Hit
TGAAAATCTTTACTCTGAAGTGGAA222220.1760060688907491No Hit
GTTGGATCCGGCCCGGACTGGCA214490.1698836365600611No Hit
CGAAAATCTTTGCTCTGAAGTGGAA201390.15950797504233627No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA181600.1438335978334985No Hit
ACCCGTAGATCCGAACTTGC178880.1416792620069175No Hit
TCAAGTCTAGGCTGGTTAGTTC176890.14010311189849972No Hit
TATTAATGCTGTAGGTGAAAGACA167380.1325708568577697No Hit
TGAAAATCTTTGCTCTGAAGTGA166690.13202435254882086No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT165440.13103430851087No Hit
CACCCCTCATCCGCTACTTTGACCTCATT162430.12865028246748436No Hit
TCTGTTTCCATATTTGAACGCTGACGAC158780.12575935387666792No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT158510.12554550436447054No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA147550.11686479823971754No Hit
TGAAAATCTTTGCTCTGAGGTGGAA146480.11601732054323162No Hit
CTTTCCGAGGTCGCGGTC145970.11561338257574767No Hit
TCCCTTTCCGAGGTCGCGGTC140620.11137599409331807No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA134450.10648913672199271No Hit
TGAAAATCCTTGCTCTGAAGTGGAA133860.1060218359360799No Hit
TTGTGTAACTCCCTAAGGAAGG126900.10050927073276962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)