FastQCFastQC Report
Thu 15 Feb 2024
sRNA-POC-48-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-48-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14848953
Total Bases365.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA319378621.50849288835381No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC10045506.765123440016276No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC2400711.6167537199424094No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1877771.2645807418206523No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1696671.1426192809688334No Hit
CTCGGGCTGAGACTTGAAGCG1423050.958350396825958No Hit
CCCTTTCCGAGGTCGCGGTC1203040.8101850682671028No Hit
TCCCTTTCCGAGGTCGCGGTC1186670.7991607219714413No Hit
CTTTCCGAGGTCGCGGTC1154240.7773207983081366No Hit
CTCCCTTTCCGAGGTCGCGGTC970110.6533187895469802No Hit
CTCGGGCTGAGACTTGAAGCA965600.6502815383683954No Hit
GGATCCGGCCCGGACTGGCC877920.5912336041470398No Hit
CTCGGGCTGAGACTTGAAGCGGTT825820.5561469552769142No Hit
GTTGGATCCGGCCCGGACTGGCC786360.5295726910846845No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA756640.5095578119211502No Hit
TGAAAATCTTTGCTCTGAAGTGGA721050.48558979208837144No Hit
TCAAGTCTAGGCTGGTTAGTTT712120.4795759000651426No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA672970.45321040480093105No Hit
TGAAAATCTTTGCTCTGAAGTGGAT575570.3876165545139782No Hit
ACCCGTAGATCCGAACTTGT574530.38691616843288545No Hit
CCTTTCCGAGGTCGCGGTC550230.37055137826889206No Hit
GGATCCGGCCCGGACTGGC531570.3579848356985169No Hit
TCTCGGGCTGAGACTTGAAGCGGTT502240.3382326013153924No Hit
TCTCGGGCTGAGACTTGAAGCG495960.3340033469026402No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC458950.3090790306899079No Hit
ACCCGTAGATCCGAACTTGTGG441350.29722634316372337No Hit
GGATCCGGCCCGGACTGGCA430350.2898184134598581No Hit
GTTGGATCCGGCCCGGACTGGCA403020.27141307538652726No Hit
GCACTGTGGTTCAGTGGTAGAATTCT381190.25671170216512906No Hit
TTCTGCTGAACTACTTTGAGAACGAGT380040.2559372367869977No Hit
TGAAAATCTTTGCTCTGAAGTGGAG368880.24842155537834887No Hit
GAGTGTGCTCTTAACTG346830.23357202356287343No Hit
TGAAAATCTTTGCTCTGAAGTGG338360.22786791769089715No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT336190.22640653519477097No Hit
TCTCGGGCTGAGACTTGAAGCA335210.22574655600297205No Hit
TTTCCGAGGTCGCGGTC316870.2133955168421639No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTC313690.2112539517095919No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC304910.2053410769095976No Hit
AGTGTGCTCTTAACTG301990.2033746082972988No Hit
TGAAAATCTTTGCTCTGAAGTGGGA269820.18170978115426725No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC268810.1810295985178214No Hit
TGAAAATCTTTGCTCTGAGGTGGAA262810.17698890958844035No Hit
CTCGGGCTGAGACTTGAAGC257450.17337922747819326No Hit
CGGACTGGCCGTGGTCGAGGC228640.1539771861356151No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG226550.1525696794918807No Hit
TCCCTTTCCGAGGTCGCGGTA222990.1501722040604479No Hit
CCCTTTCCGAGGTCGCGGTA220940.14879163534290937No Hit
CTTTCCGAGGTCGCGGTA219840.14805084237252283No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTC200330.13491186887048534No Hit
GGATCCGGCCCGGACTGGCT200170.13480411716570187No Hit
CGAAAATCTTTGCTCTGAAGTGGAA199360.1342586241602354No Hit
GTTGGATCCGGCCCGGACT197360.13291172785044172No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA194850.13122137298165062No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA191990.12929531125864563No Hit
CTCCCTTTCCGAGGTCGCGGTA188030.12662845656525412No Hit
GACCAAGATAGTCGAACTCGAC186320.12547686022038052No Hit
TGGATCCGGCCCGGACTGGCC184740.12441281213564351No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC181100.12196146085181897No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA180440.12151698506958707No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC176140.11862115800353062No Hit
GCACTGGTGGTTCAGTGGTAGAATTCA175170.11796791329328069No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG174050.11721365135979622No Hit
GTTGGATCCGGCCCGGACTGGCT172280.11602164812562878No Hit
ACCCGTAGATCCGAACTTGTGA169040.11383967610376301No Hit
CTCGGGCTGAGACTTGAAGCGGT164360.11068793873884575No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTA159170.10719274281493112No Hit
CTCGGGCTGAGACTTGAAGCGGTC153090.10309817803315831No Hit
CACTACCGATAGCCAACTCGAACGTTTCCA152270.10254595054614288No Hit
GAGTGTGCTCTTAACTA151590.10208800580081302No Hit
TGAAAATCCTTGCTCTGAAGTGGAA150990.10168393690787492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)