FastQCFastQC Report
Thu 15 Feb 2024
sRNA-POC-47-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-47-S1-TP2_R1_001.fastp-R1-31bp-auto_adapters-polyG.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12209385
Total Bases312.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA214250517.548017365330033No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2850422.3346139056144106No Hit
TGAAGATCTTTGCTCTGAAGTGGAA1349391.1052071828351715No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1173040.9607691132681948No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1011140.8281662016555298No Hit
ACCCGTAGATCCGAACTTGT964030.7895811295982557No Hit
TCAAGTCTAGGCTGGTTAGTTT908270.7439113436098542No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA880820.7214286387070274No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC873220.7152039189525107No Hit
CTCGGGCTGAGACTTGAAGCGGTT785560.6434066908365983No Hit
CCCTTTCCGAGGTCGCGGTC711620.5828467199617344No Hit
CTTTCCGAGGTCGCGGTC703930.576548286420651No Hit
CTCCCTTTCCGAGGTCGCGGTC574710.47071166975240764No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT534190.43752408495595807No Hit
ACCCGTAGATCCGAACTTGTGG505370.4139192924131723No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT483670.39614607942988117No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC480360.39343505016837454No Hit
TGAAAATCTTTGCTCTGAAGTGGAT462490.3787987683245307No Hit
TGAAAATCTTTGCTCTGAAGTGGA452100.37028892118644796No Hit
CTCGGGCTGAGACTTGAAGCG437720.35851109617724397No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC371930.3046263181970263No Hit
CTCGGGCTGAGACTTGAAGCA361250.2958789488577844No Hit
TTCCTGCACGGCTTCAGGCAATTCTGG354020.2899572746702639No Hit
GGATCCGGCCCGGACTGGC333090.27281472408315405No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA329490.26986617262048823No Hit
TGAAAATCTTTGCTCTGAAGTGGAG308920.253018477179645No Hit
ACCCGTAGATCCGAACTTGC273160.22372953265049794No Hit
TGAAAATCTTTGCTCTGAGGTGGAA250300.20500623086257005No Hit
ACCCGTAGATCCGAACTTGTGA239070.19580838838319867No Hit
TCTCGGGCTGAGACTTGAAGCGGTT232000.1900177609273522No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA226950.18588159845889043No Hit
TTCCTGCACGGCTTCAGGCAATTCTGA218970.1793456427166479No Hit
TGAAAATCTTTGCTCTGAAGTGG218350.178837836631411No Hit
GGATCCGGCCCGGACTGGCC216560.1773717513208077No Hit
CTCGGGCTGAGACTTGAAGCGGTC212590.17412015429114572No Hit
TTTCCATATTTGAACGCTGACGACAGTAC207660.17008227687143948No Hit
TCAAGTCTAGGCTGGTTAGTTC207550.16999218224341356No Hit
TGAAAATCTTTGCTCTGAAGTGGGA202470.1658314485127629No Hit
TCTGTTTCCATATTTGAACGCTGACGAC191460.15681379528944334No Hit
TTTCCATATTTGAACGCTGACGACAGTACA186640.152866012497763No Hit
AACTGCTGAGATTCTATGGATTT185140.1516374493883189No Hit
CAGCTGTGACCCTCGGGTC183370.1501877449191749No Hit
GAGTGTGCTCTTAACTG179700.14718186051140167No Hit
TTTCCATATTGAACGCTGACGACAGTACGTC169630.13893410683666704No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT168540.13804135097713766No Hit
TTCTGCTGAACTACTTTGAGAACGAGT167570.13724688016636383No Hit
AGTGTGCTCTTAACTG160690.1316118707043803No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA160400.13137434850322108No Hit
AACTTTTGACGGTGGATCT158830.1300884524486696No Hit
TTTCCGAGGTCGCGGTC157390.12890903186360328No Hit
GCACTGTGGTTCAGTGGTAGAATTCT152220.12467458434638601No Hit
TCAGTCCCACCATCTCACCAAT150320.12311840440775682No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA148110.1213083214265092No Hit
TCCCTTTCCGAGGTCGCGGTC147880.12111994174972776No Hit
CGAAAATCTTTGCTCTGAAGTGGAA144070.11799939145173978No Hit
TTGTGTAACTCCCTAAGGAAGG143770.11775367882985097No Hit
ATCCATAGAACTTCTGCATTTGA143400.11745063326285476No Hit
TAGGAATTGCCCTGGACTGCAGTAGAGGC142590.11678720918375496No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC135820.11124229434979732No Hit
TTTCCATATTTGAACGCTGACGACAGTAGG134920.11050515648413087No Hit
CTCGGGCTGAGACTTGAAGCGGT129690.1062215664425358No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT128440.1051977638513324No Hit
TTCCAAATCTGTGATGTTATTGA126470.10358425096759583No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC126300.10344501381519217No Hit
CTCGGGCTGAGACTTGAAGC125170.10251949627274427No Hit
CCCTTTCCGAGGTCGCGGTA122210.10009513173677462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)