FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-57-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-57-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574698
Total Bases385.6 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAG9751665.883461647385672No Hit
TTCCACTTCAGAGCAAAGATTTTCA7116924.293845957253641No Hit
TGCTTCAAGTCTCAGCCCGAG4726352.851545168424788No Hit
ATAGACACTCAGACAGACATGCTCC3198181.9295555188999522No Hit
GGCGAGAATTCTACCACTGAACCAC2910301.755869096378106No Hit
GGCCAGTCCGGGCCGGATCCAAC2447431.4766060895951167No Hit
AGAATTCTACCACTGAACCACCAGT1931961.1656079646217385No Hit
GAGAATTCTACCACTGAACCACCAG1782031.0751508111942674No Hit
CGCCCCAACGACCGGCAGCAGTCG1660931.0020876398471936No Hit
TCGGACTGTAGAACTCTGAACGTGT1631560.9843678599754879Illumina RNA PCR Primer (100% over 25bp)
ACCCGAGGGTCACAGCTG1477240.8912620911705299No Hit
TCCAACCGCTTCAAGTCTCAGCCCG1380290.8327693210458496No Hit
GCTTCAAGTCTCAGCCCGAG1368800.8258370680419035No Hit
AACCGCTTCAAGTCTCAGCCCGAG1175270.709074759612513No Hit
TGACAGGGTCGAGTTCGACTATCTT1108960.6690679974983557No Hit
GGCCAATCCACGGCCGATTCCTTCC1007590.6079085121188935No Hit
TGCCCCAACGACCGGCAGCAGTCG969420.584879434907351No Hit
GACAGGGTCGAGTTCGACTATCTTG962400.5806440636203447No Hit
TGCCAGTCCGGGCCGGATCCAAC953700.5753950992048241No Hit
TGCGAGAATTCTACCACTGAACCAC895170.5400822386024771No Hit
AGCTGCTCCCACTTATTCTACACCT768460.46363439020125735No Hit
GGTCTCGGCGAGGAAGAACAGCTCG734450.44311516264127404No Hit
TCATGCTCTACCGACTGAGCTAGCC711210.4290937910301594No Hit
AGCACTGCAAGCAGTCCACCACG663800.400489951611788No Hit
CCGCTTCAAGTCTCAGCCCGAG637890.38485769092142735No Hit
AGCCAGTCCGGGCCGGATCCAAC595070.3590231327291755No Hit
AGAATTCTACCACTGAACCACAGTG587540.354480063528156No Hit
AGCCTTAACTCCAGAAAGTG583320.3519340141220069No Hit
AGCGAGAATTCTACCACTGAACCAC572950.34567748987040364No Hit
AGCCGGGCTGTAACAGTCCGGAGCC565400.34112235408452085No Hit
GAGAATTCTACCACTGAACCACAGT558620.3370317818158738No Hit
GGGCCGGATCCAACCGCTTCAAGTC539920.3257495249687204No Hit
ATCCAACCGCTTCAAGTCTCAGCCC490300.2958123279229582No Hit
GCCTCGACCACGGCCAGTCCG487970.29440657078638777No Hit
CGGTCCGACCGCGACCTCGGAA474890.28651502428581205No Hit
ACCTCGACCACGGCCAGTCCG471500.2844697381514885No Hit
GACCGCGACCTCGGAAAG470990.2841620402374752No Hit
CGGTCTCGGCGAGGAAGAACAGCTC459620.2773021867427087No Hit
TGCCAATCCACGGCCGATTCCTTCC459350.2771392878470546No Hit
GTCTCGGCGAGGAAGAACAGCTCGG403550.24347351607854334No Hit
GCCCCAACGACCGGCAGCAGTCG400890.24186866029172902No Hit
CGGAAACGTTCGAGTTGGCTATCGG399520.2410420992285953No Hit
GGCCGGATCCAACCGCTTCAAGTCT388900.2346347426662012No Hit
CGAGAATTCTACCACTGAACCACCA383270.23123799902719194No Hit
AAACTAACCAGCCTAGACTTGA363760.21946704549307625No Hit
AGGCGAGAATTCTACCACTGAACCA349070.2106041389110076No Hit
GGAATTCTACCACTGAACCACCAGT348850.2104714064775117No Hit
GACCCGAGGGTCACAGCTG347640.2097413780932841No Hit
ATCCAACCGCTTCAAGTCT334000.2015119672165369No Hit
GCCCGAGGGTCACAGCTG332530.20062507322908688No Hit
TGGTCTCGGCGAGGAAGAACAGCTC328500.19819365637913888No Hit
AGCACACTCGGTCTCGGCGAGGAAG324680.1958889386702551No Hit
AGTCCGGGCCGGATCCAAC324200.19559934063353673No Hit
AGCCTTAACTCCAGAAAGTGA321020.19368075364027748No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG317160.19135190276166722No Hit
TGGTCCGACCGCGACCTCGGAA314470.189728947097558No Hit
AGGGAGAGAGAACCCCCCCTCACCT314200.18956604820190387No Hit
TGGCCGGATCCAACCGCTTCAAGTC309680.18683900002280585No Hit
CGCTTCAAGTCTCAGCCCGAGA309650.18682090014551095No Hit
GCCAGTCCGGGCCGGATCCAAC303440.18307422554546696No Hit
TGCTGCTCCCACTTATTCTACACCT290990.17556277646808408No Hit
CGCCCCAACGACCGGCAGCAGTC276550.16685070219680626No Hit
AGCCAATCCACGGCCGATTCCTTCC260300.15704660199540285No Hit
TGGGCCAATCCACGGCCGATTCCTT259950.15683543676029574No Hit
CAACCGCTTCAAGTCTCAGCCCGAG259150.1563527733657651No Hit
AGCTTCAAGTCTCAGCCCGAG240320.14499208371700045No Hit
TGCCGGGCTGTAACAGTCCGGAGCC238100.14365269279717796No Hit
GTAGACACTCAGACAGACATGCTCC235960.14236156821680854No Hit
TGCCTTAACTCCAGAAAGTG232740.14041884805382276No Hit
TGGAAACGTTCGAGTTGGCTATCGG231690.1397853523485013No Hit
TGCACTGCAAGCAGTCCACCACG230070.1388079589745768No Hit
CAGTTAAGAGCACACTCG227750.13740823513043796No Hit
ACAAGTTCGGATCTACGGGT227260.13711260380128795No Hit
GAAACGTTCGAGTTGGCTATCGGTA225830.13624984298356446No Hit
TGTCTCGGCGAGGAAGAACAGCTCG220390.1329677319007562No Hit
GCGAGCTGCTCCCACTTATTCTACA217430.13118187734099288No Hit
GACCGCTTCAAGTCTCAGCCCGAG216140.13040358261731225No Hit
ACCGCTTCAAGTCTCAGCCCGAG214950.12968562081794793No Hit
AGCACTGCAAGCAGTCCACCACGGT210840.12720593762854684No Hit
ATCCACTTCAGAGCAAAGATTTTCA210730.12713957141179888No Hit
TCGCTTCAAGTCTCAGCCCGAG204480.12336876364202834No Hit
AGCCTTAACTCCAGAAAG203030.12249393623944159No Hit
CCTCGACCACGGCCAGTCCG196280.11842146384808944No Hit
TGACAGGGTCGAGTTCGA196130.11833096446161494No Hit
GTCGAGTTCGACTATCTTGGTC195610.11801723325517001No Hit
ACTTCAAGTCTCAGCCCGAG193010.11644857722294549No Hit
ATCCAACCGCTTCAAGTC191970.11582111481005566No Hit
TGAGAATTCTACCACTGAACCACCA189330.11422832560810459No Hit
TGCCGGATCCAACCGCTTCAAGTCT188220.11355863014819334No Hit
GGCTGCTCCCACTTATTCTACACCT187960.1134017645449709No Hit
GCCAATCCACGGCCGATTC184060.11104878049663408No Hit
GGTCCGACCGCGACCTCGGAA182590.11016188650918406No Hit
TCACCTCGACCACGGCCAGTCCG182580.11015585321675243No Hit
TCCCGGTCCGACCGCGACCTCGGAA180100.10865959669370748No Hit
CGGTCCGACCGCGACCTCGGAAAGG176780.10665654360640538No Hit
AGGCCGGATCCAACCGCTTCAAGTC175150.10567311694004923No Hit
GGCCAGTCCGGGCCGGATCCAACCG173060.10441215882183796No Hit
TCGGTCTCGGCGAGGAAGAACAGCT172410.10401999481378181No Hit
TGAATTCTACCACTGAACCACCAGT172080.10382089616353796No Hit
CCACAAGTTCGGATCTACGGGT172060.10380882957867468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)