FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-57-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-57-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574698
Total Bases385.4 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG9835165.933839639189806No Hit
GCACTGGTGGTTCAGTGGTAGAATT8658955.224197750088719No Hit
TGAAAATCTTTGCTCTGAAGTGGAA7157084.318075659659078No Hit
CTCGGGCTGAGACTTGAAGCA4734462.8564381685868425No Hit
CTCGGGCTGAGACTTGAAGCGGTTG3595562.169306493548178No Hit
AACTTTTGACGGTGGATCTCTAGGC3251551.961755200607577No Hit
GCACTGTGGTTCAGTGGTAGAATTC2726371.644898748683083No Hit
GTTGGATCCGGCCCGGACTGGCC2500911.5088721375194891No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC1685001.0166097747301337No Hit
CGACTGCTGCCGGTCGTTGGGGCG1658701.0007422156349395No Hit
CGACCAAGATAGTCGAACTCGACCC1614270.9739362973611948No Hit
GGAAGAGCACACGTCTGAACTCCAG1601080.9659783846438711Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTGTAGAATAAGTGGGAGCAGC1575160.9503400906610787No Hit
CAGCTGTGACCCTCGGGT1511020.9116425530045857No Hit
CTCGGGCTGAGACTTGAAGC1415000.853710879076047No Hit
GCCGAGCTGTTCTTCCTCGCCGAGA1317040.7946087464157718No Hit
CTCGGGCTGAGACTTGAAGCGGTT1179520.7116389088959569No Hit
GGAAGGAATCGGCCGTGGATTGGCC1032740.6230822425844501No Hit
GTTGGATCCGGCCCGGACTGGCA952400.5746107711887118No Hit
CGACTGCTGCCGGTCGTTGGGGCA949100.572619784686273No Hit
CTGGAGTCTCGGCTCCGGACTGTTA876060.5285526167656268No Hit
CCGGGAAGGAATCGGCCGTGGATTG743870.4487985241118722No Hit
CGTGGTGGACTGCTTGCAGTGCT669710.404055627438883No Hit
CTCGGGCTGAGACTTGAAGCGG641630.38711414229085805No Hit
AGACTTGAAGCGGTTGGATCCGGCC640180.38623931488827123No Hit
GTTGGATCCGGCCCGGACTGGCT596830.3600849921971429No Hit
CACTTTCTGGAGTTAAGGCT588090.3548118946118958No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC587330.3543533643870917No Hit
CACTACCGATAGCCAACTCGAACGT569870.3438192358014608No Hit
CCGACCAAGATAGTCGAACTCGACC567210.34221438001464644No Hit
CGGACTGGCCGTGGTCGAGGC495490.29894360669497566No Hit
TTCCGAGGTCGCGGTCGGACCG485290.29278964841471017No Hit
CTTTCCGAGGTCGCGGTC480870.29012293315992843No Hit
CGGACTGGCCGTGGTCGAGGT472740.285217866413011No Hit
CGGACTGGCCGTGGTCGAGGTGAGG454020.2739235429809943No Hit
CAACTTTTGACGGTGGATCTCTAGG445430.26874094478222166No Hit
GACCAAGATAGTCGAACTCGACCCT427470.2579051515750091No Hit
CGACTGCTGCCGGTCGTTGGGGC405800.24483100687566073No Hit
TCAAGTCTAGGCTGGTTAGTTT364480.2199014425481538No Hit
CAGCTGTGACCCTCGGGTC360490.21749415886793233No Hit
GACTTGAAGCGGTTGGATCCGGCCC352080.21242015993292912No Hit
CCCGGCTAGCTCAGTCGGTAGAGCA349140.21064637195802904No Hit
CGTGGTCGAGGTGAGGGGGGGTTCT345140.20823305498537592No Hit
CAGCTGTGACCCTCGGGC344600.20790725719406775No Hit
AGACTTGAAGCGGTTGGAT337130.20340038774763802No Hit
TTCCGAGGTCGCGGTCGGACCGGGA333200.2010293038220063No Hit
GTTGGATCCGGCCCGGACT327370.19751189433436434No Hit
TCACTTTCTGGAGTTAAGGCT323630.19525544296493366No Hit
TTCCGAGGTCGCGGTCGGACCA318710.1922870630885703No Hit
GTTGGATCCGGCCCGGACTGGC314800.18992804574780184No Hit
TCTCGGGCTGAGACTTGAAGCG311740.1880818582637222No Hit
GACTGCTGCCGGTCGTTGGGGCG276640.16690500182869095No Hit
TCTCGGGCTGAGACTTGAAGCGGTT265720.16031664649334787No Hit
GCACTACCGATAGCCAACTCGAACG262060.15810846146337026No Hit
TTCCTGCACGGCTTCAGGCAATTCT261910.15801796207689575No Hit
GCACTTGTGGTTCAGTGGTAGAATT242880.14653660657949846No Hit
CTCGGGCTGAGACTTGAAGCT239500.14449735373760655No Hit
GAATCGGCCGTGGATTGGCCCAGCT234650.14157120690826464No Hit
CACTTTCTGGAGTTAAGGCA232760.140430914638686No Hit
CGTGGTGGACTGCTTGCAGTGCA230280.1389346581156411No Hit
ACCCGTAGATCCGAACTTGT229760.1386209269091962No Hit
CGAGTGTGCTCTTAACTG229440.13842786155138392No Hit
GGAAGGAATCGGCCGTGGATTGGCA222750.13439158891462155No Hit
CTCGGGCTGAGACTTGAAGCGGTC219590.13248506850622557No Hit
CTCGGGCTGAGACTTGAAGCGGT216350.13053028175837653No Hit
CTCGGGCTGAGACTTGAAGCGA213300.1286901275667285No Hit
TGAAAATCTTTGCTCTGAAGTGGAT206910.12483485370291512No Hit
CTTTCTGGAGTTAAGGCT204470.12336273034959672No Hit
GAGACTTGAAGCGGTTGGATCCGGC202570.12221640478758647No Hit
GACCAAGATAGTCGAACTCGAC201590.12162514212928645No Hit
CGGACTGGCCGTGGTCGAGG198980.1200504528046303No Hit
TCGAACTCGACCCTGTCA198380.11968845525873231No Hit
GACTTGAAGCGGTTGGAT193400.11668387562777915No Hit
TTCCGAGGTCGCGGTCGGACC190660.11503075350151176No Hit
CTCGGGCTGAGACTTGAAGT190380.11486182131342604No Hit
GAATCGGCCGTGGATTGGC185590.11197187423867391No Hit
CGGACTGGCCGTGGTCGAGGTGA183710.11083761526152693No Hit
TGATGACTCTTTTCTATTCTGACGA180900.1091422600882381No Hit
GTTCTATTTTGTTGGTTTCTGGAAC179810.10848463121319013No Hit
GAACTGGGCGTCGGAGGTGCCGTGG179710.1084242982888738No Hit
CGAGCTGTTCTTCCTCGCCGAGACC179700.10841826499644217No Hit
ACCCGTAGATCCGAACTTGTGG178890.10792956830947992No Hit
TCCTGGTGTCTATGACGTAGTGGAA172150.10386312921055937No Hit
ACACTGGTGGTTCAGTGGTAGAATT168830.10186007612325727No Hit
GGCCGAGCTGTTCTTCCTCGCCGAG167000.10075598360826846No Hit
TGAAAATCTTTGCTCTGAAGTGGA166090.10020695399698987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)