FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-53-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-53-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18481608
Total Bases437.4 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAG16575998.968911146692431No Hit
TTCCACTTCAGAGCAAAGATTTTCA9526185.154410806678726No Hit
TTCCACCTCAGAGCAAAGATTTTCA5585843.0223777065285664No Hit
TGCTTCAAGTCTCAGCCCGAG4922722.663577757952663No Hit
ATAGACACTCAGACAGACATGCTCC4383122.3716118207896195No Hit
TCGGACTGTAGAACTCTGAACGTGT2389241.2927663004214784Illumina RNA PCR Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCAC2353551.2734552101743528No Hit
GGCCAGTCCGGGCCGGATCCAAC1347110.7288922046176934No Hit
AGAATTCTACCACTGAACCACCAGT1049810.5680295783786778No Hit
AACCGCTTCAAGTCTCAGCCCGAG1047420.5667364008586265No Hit
GCTTCAAGTCTCAGCCCGAG1036970.5610821309487789No Hit
CGCTTCAAGTCTCAGCCCGAGA1025860.5550707492551513No Hit
GCCTCGACCACGGCCAGTCCG1018720.5512074490488057No Hit
GAGAATTCTACCACTGAACCACCAG939190.5081754791033334No Hit
CTCCACTTCAGAGCAAAGATTTTCA848320.45900767941837095No Hit
CGCCCCAACGACCGGCAGCAGTCG807480.43691003510084186No Hit
GGCCAATCCACGGCCGATTCCTTCC794880.4300924465014083No Hit
CCGCTTCAAGTCTCAGCCCGAG763750.41324867403312526No Hit
CCACAAGTTCGGATCTACGGGT725800.3927147464657837No Hit
TGCGAGAATTCTACCACTGAACCAC708160.3831701224265767No Hit
TGACAGGGTCGAGTTCGACTATCTT698040.37769440840861895No Hit
CGAGAATTCTACCACTGAACCACCA682950.36952953444310693No Hit
AAACTAACCAGCCTAGACTTGA584460.31623871689086797No Hit
CGGAAACGTTCGAGTTGGCTATCGG575280.31127161662556635No Hit
CGTTTCAAGTCTCAGCCCGAG565920.30620712223741575No Hit
CGGTCTCGGCGAGGAAGAACAGCTC554630.30009834642093913No Hit
CTCCACCTCAGAGCAAAGATTTTCA550820.29803683748730087No Hit
TGCCAGTCCGGGCCGGATCCAAC534070.28897377327773643No Hit
CGGTCCGACCGCGACCTCGGAAAGG522350.2826323337233427No Hit
GACAGGGTCGAGTTCGACTATCTTG517840.2801920698675137No Hit
TCCACAGAATTATTTAAAAGTGATG517340.27992153063737746No Hit
TCCAACCGCTTCAAGTCTCAGCCCG479500.25944712170066586No Hit
ACAAGTTCGGATCTACGGGT456510.24700772789900102No Hit
TCATGCTCTACCGACTGAGCTAGCC454690.24602296510130503No Hit
GGTCTCGGCGAGGAAGAACAGCTCG405380.21934238622526783No Hit
AGCGAGAATTCTACCACTGAACCAC402690.2178868851671348No Hit
GACCGCGACCTCGGAAAG401740.21737286062987593No Hit
CCAGAATTGCCTGAAGCCGTGCAGG372550.2015787803745215No Hit
GTAGACACTCAGACAGACATGCTCC361630.1956702035883458No Hit
ACCCGAGGGTCACAGCTG347540.1880464080831062No Hit
AGGCGAGAATTCTACCACTGAACCA345170.18676405213226036No Hit
TGCCAATCCACGGCCGATTCCTTCC336410.1820242048202732No Hit
GCCAGAATTGCCTGAAGCCGTGCAG323710.1751525083748124No Hit
CAACCGCTTCAAGTCTCAGCCCGAG314980.17042889341663345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG311490.1685405295902824No Hit
AGAATTCTACCACTGAACCACAGTG310710.16811848839126986No Hit
TGCTTCAAGTCTCAGCCCGAGA307550.16640868045680873No Hit
ACAAATCTTAGAACAAAGGCTTAAT304900.1649748225370866No Hit
TGCCCCAACGACCGGCAGCAGTCG299750.1621882684666832No Hit
AGCCAGTCCGGGCCGGATCCAAC290530.15719952506297072No Hit
GAGAATTCTACCACTGAACCACAGT283040.15314684739552964No Hit
AGCACACTCGGTCTCGGCGAGGAAG276060.14937011974282757No Hit
AGAGAGAACCCCCCCTCGCCTCGAC275500.14906711580507495No Hit
CCACTTCAGAGCAAAGATTTTCA274840.1487100040212951No Hit
AGCCGGGCTGTAACAGTCCGGAGCC272530.14746011277806564No Hit
CCACCTCAGAGCAAAGATTTTCA272110.14723285982475118No Hit
ACAGGGTCGAGTTCGACTATCTTGG267800.14490081166097668No Hit
AGCTGCTCCCACTTATTCTACACCT267030.14448418124656687No Hit
TTCCCGGTCCGACCGCGACCTCGGA261890.1417030379607662No Hit
CGGCGAGAATTCTACCACTGAACCA253810.13733112400176434No Hit
CGCGACCTCGGAAAGGGAGAGAGAA251770.13622732394280843No Hit
GGAGAGAGAACCCCCCCTCGCCTCG244800.1324560070747091No Hit
AGATTAAGAGTCTCATGCTCTACCG239070.12935562749734764No Hit
ATCCAACCGCTTCAAGTCTCAGCCC236220.127813553885571No Hit
GCCAGTCCGGGCCGGATCCAAC222850.12057933487172762No Hit
ATCCACTTCAGAGCAAAGATTTTCA220820.11948094559737442No Hit
TTTTTTTGGTCTTTCACCTACAGCA219170.1185881661379248No Hit
GCCAGTCCGGGCCGGATCC218420.11818235729272042No Hit
GGGCCGGATCCAACCGCTTCAAGTC217690.11778737001672149No Hit
GACCGCGACCTCGGAAAGGG210170.11371845999547225No Hit
GACCGCTTCAAGTCTCAGCCCGAG209930.11358860116500685No Hit
CGCGAGAATTCTACCACTGAACCAC209070.1131232736891725No Hit
CGAGAATTCTACCACTGAACCACAG209000.11308539819695343No Hit
GGAATTCTACCACTGAACCACCAGT204950.11089403043284979No Hit
TGGTCTCGGCGAGGAAGAACAGCTC204380.11058561571049447No Hit
GGCCAGTCCGGGCCGGATCC201720.1091463470061696No Hit
TCCACTTCAGAGCAAAGATTTTCA200590.10853492834606167No Hit
TCCACCTCAGAGCAAAGATTTTCA198070.10717141062617495No Hit
GACCGCGACCTCGGAAAGGGA192410.10410890654103258No Hit
CCTTCCTTAGGGAGTTACACAA191830.10379508103407452No Hit
AACCGCTTCAAGTCTCAGCCCGAGA187450.10142515737808096No Hit
ACCTCGACCACGGCCAGTCCG187350.10137104953205371No Hit
CGAATTCTACCACTGAACCACCAGT185630.100440394580385No Hit
GCCTCGACCACGGCCAGTCC185290.10025642790389236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)