FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-53-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-53-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18481608
Total Bases437.2 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG16677449.023803556487076No Hit
TGAAAATCTTTGCTCTGAAGTGGAA9545365.164788691546753No Hit
GCACTGGTGGTTCAGTGGTAGAATT6757493.656332284506846No Hit
TGAAAATCTTTGCTCTGAGGTGGAA5602113.0311810530771996No Hit
CTCGGGCTGAGACTTGAAGCA4929172.6670677140214205No Hit
AACTTTTGACGGTGGATCTCTAGGC4564802.469914955451928No Hit
GGAAGAGCACACGTCTGAACTCCAG2373761.2843904058564601Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTC2052481.11055271814011No Hit
CTCGGGCTGAGACTTGAAGCGGTTG1703500.9217271570742113No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC1628090.8809244303850617No Hit
CGACCAAGATAGTCGAACTCGACCC1468720.7946927561714328No Hit
GTTGGATCCGGCCCGGACTGGCC1377880.7455411888402784No Hit
CTCGGGCTGAGACTTGAAGC1077580.5830553272204453No Hit
CTCGGGCTGAGACTTGAAGCGGTT1036850.5610172015335462No Hit
CGGACTGGCCGTGGTCGAGGC1035900.5605031769962874No Hit
TCTCGGGCTGAGACTTGAAGCG1031650.5582035935401292No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC951850.5150255324103834No Hit
CGGACTGGCCGTGGTCGAGGCGAGG925850.5009574924432982No Hit
TTCCTGCACGGCTTCAGGCAATTCT861040.4658901974330372No Hit
CGACTGCTGCCGGTCGTTGGGGCG780980.42257145590362044No Hit
CTCGGGCTGAGACTTGAAGCGG767590.4153264153205717No Hit
TGAAAATCTTTGCTCTGAAGTGGAG742510.40175616753693727No Hit
TCTTGCATCACTTTTAAATAATTCT677780.366732158803498No Hit
GGAAGGAATCGGCCGTGGATTGGCC662400.35841037208450693No Hit
CAACTTTTGACGGTGGATCTCTAGG655540.35469857384703757No Hit
GCCGAGCTGTTCTTCCTCGCCGAGA655320.3545795365857776No Hit
GAGGTGTAGAATAAGTGGGAGCAGC617550.3341430031412851No Hit
CCGGGAAGGAATCGGCCGTGGATTG615650.3331149540667674No Hit
TCAAGTCTAGGCTGGTTAGTTT580860.31429083443388695No Hit
CTCGGGCTGAGACTTGAAACG567450.3070349722816326No Hit
ACCCGTAGATCCGAACTTGTGG559250.3025981289073981No Hit
GTTGGATCCGGCCCGGACTGGCA532900.2883407114792176No Hit
TGAAAATCTTTGCTCTGAGGTGGAG503800.2725953282852877No Hit
CTGGAGTCTCGGCTCCGGACTGTTA495810.2682721113877104No Hit
TGATCATGGATTCTAAACTGGTAAT461640.24978346040019894No Hit
ACCCGTAGATCCGAACTTGT447280.24201357371068577No Hit
TATTAATGCTGTAGGTGAAAGACCA419700.2270906297763701No Hit
CTTTCCGAGGTCGCGGTC410920.22233996089517755No Hit
CACTACCGATAGCCAACTCGAACGT401390.21718348316878056No Hit
GTGGTCCGGACGGTCGAGGGTTATC393790.21307128687070953No Hit
CCGACCAAGATAGTCGAACTCGACC378550.20482525113615654No Hit
CCCTTTCCGAGGTCGCGGTCGGACC374240.20249320297238207No Hit
CTTTCCGAGGTCGCGGTCGGACCGG357290.1933219230707631No Hit
CAGCTGTGACCCTCGGGT355340.19226682007323173No Hit
CACCCCTCATCCGCTACTTTGACCT349630.18917726206507574No Hit
GTGGTCGAGGCGAGGGGGGGTTCTC348560.18859830811258416No Hit
GAGGTCCGGACGGCTGAGGGTTATC320620.17348057593257038No Hit
TAGAATGTTTACTCGTGAAGGAAAA316300.17114311698419316No Hit
TCTCGGGCTGAGACTTGAAGCA307580.16642491281061692No Hit
TCTCGGGCTGAGACTTGAAGCGGTT295580.15993197128734687No Hit
CGCCCCTCCCCGCCCTTTGACCTCA292120.15805983981480398No Hit
GTTGGATCCGGCCCGGACTGGCT291340.15763779861579144No Hit
CGACTGCTGCCGGTCGTTGGGGCA285470.15446166805399184No Hit
TGTGACTTCTCTATCCGCACGCGGC284180.1537636768402403No Hit
TCTTCGTACTGTTTCCAAACGTTCT274380.14846110792956976No Hit
CACCCCTCGCCTCTACTTGACCTCA264020.14285553508114662No Hit
TACCAAGGTCTTGTAGTCTAGTACA242740.13134138544654772No Hit
GGACTGGCCGTGGTCGAGGCGAGGG241550.13069750207882344No Hit
TGAAAATCTTTGCTCTGAAGTGG239100.12937185985115582No Hit
TGAAAATCTTTGCTCTGAGGTGG233150.12615244301253442No Hit
CGGTGCAGGAATTAACTGAGTTGGA232080.12557348906004284No Hit
GTTGGATCCGGCCCGGACTGGC230010.12445345664727873No Hit
TTGTCTACAAGAAGCCTGTCCATTT224860.12166690257687535No Hit
GGATCCGGCCCGGACTGGC223030.12067672899457667No Hit
TGCGTTTCTGGTAGACTTATGTTTT215860.1167971964344228No Hit
CCCTTTCCGAGGTCGCGGTC214400.11600722188242496No Hit
TGAAAATCTTTGCTCTGAAGTGGAT214000.11579079049831595No Hit
CTCGGGCTGAGACTTGAAGCGGTC212800.11514149634598894No Hit
GCACTTGTGGTTCAGTGGTAGAATT210780.11404851785623848No Hit
TCCCTTTCCGAGGTCGCGGTCGGAC209600.11341004527311693No Hit
TGAAAATCTTTGCTCTGAAGTGGA207530.11229001286035284No Hit
GGATCCGGCCCGGACTGGCC205940.11142969810851956No Hit
TGAAAATCTTTGCTCTGAGGTGGA203140.10991467841975655No Hit
TCCCTTTCCGAGGTCGCGGTC196700.10643013313560161No Hit
CCGGCCCGGACTGGCCGTGGTCGAG195500.1057808389832746No Hit
TTGCTACGATCTTCTGAGATTAAGC193230.10455259087845603No Hit
GAGGTCCGGATTAGGGGAGGGTTAC192310.10405479869500533No Hit
ACCCGTAGATCCGAACTTGGGG191770.10376261632645818No Hit
GGACTGGCCGTGGTCGAGGC187740.10158207013155997No Hit
TGTCCACCCCCCACCGGCTGTTTTT187240.10131153090142374No Hit
CAGCTGTGACCCTCGGGTC186770.10105722402509565No Hit
CGGACTGGCCGTGGTCGAGGT186190.1007433985181376No Hit
TAGGAATTGCCCTGGACTGCAGTAG185040.10012115828882422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)