FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-50-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-50-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14858812
Total Bases361.1 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA312590421.037374993370936No Hit
ATAGACACTCAGACAGACATGCTCC6824184.592682106752545No Hit
TCGGACTGTAGAACTCTGAACGTGT5480063.6880875806221924Illumina RNA PCR Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCAC4567883.074189242047076No Hit
TGCGAGAATTCTACCACTGAACCAC1744741.1742123125321189No Hit
GAGAATTCTACCACTGAACCACCAG1301280.8757631498399738No Hit
CGCTTCAAGTCTCAGCCCGAG1195220.8043846304805525No Hit
AAACTAACCAGCCTAGACTTGA994840.6695286271876918No Hit
TGCTTCAAGTCTCAGCCCGAG878200.5910297539264916No Hit
AGCGAGAATTCTACCACTGAACCAC877120.5903029125074064No Hit
ACAAGTTCGGATCTACGGGT751430.5057133773547979No Hit
TCCACTTCAGAGCAAAGATTTTCA654490.44047262997876274No Hit
ATCCACTTCAGAGCAAAGATTTTCA639810.43059297068971597No Hit
GTAGACACTCAGACAGACATGCTCC608770.40970300990415653No Hit
CCACAAGTTCGGATCTACGGGT568190.3823926165833446No Hit
GCCTCGACCACGGCCAGTCCG533560.3590865810806409No Hit
GCCAGAATTGCCTGAAGCCGTGCAG495840.3337009715177768No Hit
ACAAATCTTAGAACAAAGGCTTAAT484920.3263517971692488No Hit
AGGCGAGAATTCTACCACTGAACCA454170.30565700676474No Hit
AACCGCTTCAAGTCTCAGCCCGAG447260.30100656768522277No Hit
CTCCACTTCAGAGCAAAGATTTTCA445850.3000576358325282No Hit
AGAATTCTACCACTGAACCACCAGT440320.2963359385662864No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG429540.2890809844017139No Hit
TCCAACCGCTTCAAGTCTCAGCCCG419030.2820077405919127No Hit
GGCCAGTCCGGGCCGGATCCAAC379660.2555116788610018No Hit
CCACTTCAGAGCAAAGATTTTCA370270.24919219652284447No Hit
GAGAATTCTACCACTGAACCACAGT354990.23890873644541702No Hit
TTTTTTTGGTCTTTCACCTACAGCA328280.22093287134933803No Hit
CCAGAATTGCCTGAAGCCGTGCAGG301620.2029906563189574No Hit
TCCCACTTCAGAGCAAAGATTTTCA275860.18565414247114775No Hit
TGGCGAGAATTCTACCACTGAACCA267950.18033070207766275No Hit
AATGAGGTCAAAGTAGCGGATGAGG253900.17087503361641565No Hit
TTCCACTTCAGAGTAAAGATTTTCA224270.15093400468355073No Hit
TCACAAGTTCGGATCTACGGGT218840.14727960754870578No Hit
TGCCAGTCCGGGCCGGATCCAAC216350.14560383427692605No Hit
TGACAGGGTCGAGTTCGACTATCTT215690.1451596534097073No Hit
TCATGCTCTACCGACTGAGCTAGCC207260.13948625233295905No Hit
TTCCACTTCAGAGCAAAGATTTTCG205780.13849021038828677No Hit
GGCCAATCCACGGCCGATTCCTTCC188270.1267059573807112No Hit
GACAGGGTCGAGTTCGACTATCTTG186020.12519170442428373No Hit
GAACTAACCAGCCTAGACTTGA176900.1190539324408977No Hit
GCAAGTTCGGATCTACGGGT176370.11869724174449478No Hit
TGTCTTTCACCTACAGCATTAATA168930.11369011196857462No Hit
TCACTTCAGAGCAAAGATTTTCA167750.11289597041809264No Hit
ACTCATCCTAAGTAGACTTTTCACA165200.11117981706747485No Hit
GTCGTCAGCGTTCAAATATGGAAAC164880.11096445664700516No Hit
ACAGACCGAAAAACACAGGATGAAT161820.1089050726262638No Hit
GGGCCGGATCCAACCGCTTCAAGTC151950.10226254965740195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)