FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-50-S1-TP2.flexbar_trim.25bp_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-50-S1-TP2.flexbar_trim.25bp_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14858812
Total Bases361 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA314177521.14418703191076No Hit
GCACTGGTGGTTCAGTGGTAGAATT8560935.761517138786062No Hit
AACTTTTGACGGTGGATCTCTAGGC6868684.622630665224111No Hit
GGAAGAGCACACGTCTGAACTCCAG5392513.629166315584315Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTC2249121.5136607152711805No Hit
TTCCTGCACGGCTTCAGGCAATTCT1232520.8294875794915502No Hit
CTCGGGCTGAGACTTGAAGCG1210600.8147353906893768No Hit
CAACTTTTGACGGTGGATCTCTAGG1046280.7041478147781935No Hit
CTCGGGCTGAGACTTGAAGCGGTTG1019860.6863671200631651No Hit
TCAAGTCTAGGCTGGTTAGTTT998670.6721062222201882No Hit
CTCGGGCTGAGACTTGAAGCA879460.5918777355820909No Hit
ACCCGTAGATCCGAACTTGT761400.5124232004550566No Hit
TGAAAATCTTTGCTCTGAAGTGGA679060.4570082722629508No Hit
TATTAATGCTGTAGGTGAAAGACCA630380.424246568299No Hit
TGAAAATCTTTGCTCTGAAGTGGAT624060.4199931999947237No Hit
ACCCGTAGATCCGAACTTGTGG591130.39783126672576513No Hit
CGGACTGGCCGTGGTCGAGGC543370.36568872397066465No Hit
TAGAATGTTTACTCGTGAAGGAAAA504710.3396704931726709No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC473170.31844403173012753No Hit
CTCGGGCTGAGACTTGAAGCGGTT449500.30251409062851053No Hit
TGAAAATCTTTGCTCTGAAGTGGAG448950.3021439399058283No Hit
CACCCCTCATCCGCTACTTTGACCT412330.27749863178832873No Hit
GTTGGATCCGGCCCGGACTGGCC385770.2596237168893449No Hit
TATAGACTTGGAAGCGGGAAAAAAA374350.25193804188383295No Hit
CGACCAAGATAGTCGAACTCGACCC373310.2512381205173065No Hit
TGAAAATCTTTGCTCTGAAGTGG373190.2511573603596304No Hit
TTGTTTATGCCGTGAGGATTGGAGG301170.20268780572767192No Hit
TGTGACTTCTCTATCCGCACGCGGC293990.19785565629338334No Hit
TGAAAATCTTTGCTCTGAAGTGGGA283690.19092374275951535No Hit
TCTGTTTCCATATTTGAACGCTGAC274250.18457061035565966No Hit
GCACTTGTGGTTCAGTGGTAGAATT261040.1756802629981455No Hit
TCCCGATCGTTGTTAAAGTCTTAGA254460.17125191435223758No Hit
TGATCTGCGCATAGGAATTGCCCTG241740.1626913376385676No Hit
TGATGACTCTTTTCTATTCTGACGA228620.1538615603993105No Hit
ACCCGTAGATCCGAACTTGTGA226920.1527174581655653No Hit
TGAAAATCTTTACTCTGAAGTGGAA225430.15171468620775336No Hit
TGGTCTCTGAATGTAATATTTGTAA223870.15066480415796363No Hit
TTCAATGTGAAAAGTCTACTTAGGA220170.1481746992962829No Hit
ACACTGGTGGTTCAGTGGTAGAATT218570.14709789719393448No Hit
GTTGGATCCGGCCCGGACTGGCA216290.14556345419808797No Hit
GATACGATCCACTGAGATTCATCCT208490.1403140439491394No Hit
CGAAAATCTTTGCTCTGAAGTGGAA204800.13783066910059835No Hit
GAGGTCCGGACGGCTGAGGGTTATC197640.13301197969258916No Hit
TTGCTACGATCTTCTGAGATTAAGC194390.13082472542219392No Hit
CGGACCTCAGATCAGGCAAGGCTAC192680.12967389317530906No Hit
GTGGTCCGGACGGTCGAGGGTTATC183700.1236303413758785No Hit
TGTCCACCCCCCACCGGCTGTTTTT182870.12307175028528526No Hit
GTTGCTACGATCTTCTGAGATTAAG181570.12219684857712716No Hit
ACCCGTAGATCCGAACTTGC181420.12209589838003199No Hit
TCAAGTCTAGGCTGGTTAGTTC179420.12074989575209646No Hit
CGGACTGGCCGTGGTCGAGGCGAGG179330.12068932563383938No Hit
GCCGAGCTGTTCTTCCTCGCCGAGA174480.1174252692610957No Hit
CACCAAGGTCTTGTAGTCCAGTACA174160.11720990884062601No Hit
TATTAATGCTGTAGGTGAAAGACA169770.11425543307230752No Hit
CCGGGAAGGAATCGGCCGTGGATTG168950.11370357199485395No Hit
TGAAAATCTTTGCTCTGAAGTGA168080.11311806085170201No Hit
GGAAGGAATCGGCCGTGGATTGGCC160930.10830610145683248No Hit
TCTCGGGCTGAGACTTGAAGCGGTT160730.10817150119403893No Hit
CTTTCCGAGGTCGCGGTC148850.10017624558410188No Hit
TGAAAATCTTTGCTCTGAGGTGGAA148660.100048375334448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)