FastQCFastQC Report
Fri 17 Nov 2023
sRNA-POC-48-S1-TP2.flexbar_trim.25bp_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamesRNA-POC-48-S1-TP2.flexbar_trim.25bp_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574535
Total Bases397.5 Mbp
Sequences flagged as poor quality0
Sequence length18-25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCA321975019.42588434607668No Hit
GAGAATTCTACCACTGAACCACCAG10419646.286535338698792No Hit
ATAGACACTCAGACAGACATGCTCC5898893.5590078394356164No Hit
TCGGACTGTAGAACTCTGAACGTGT4368522.6356817853411876Illumina RNA PCR Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCAC2507231.5127000546320002No Hit
GAGAATTCTACCACTGAACCACAGT2492851.5040240947936097No Hit
AGAATTCTACCACTGAACCACCAGT1759841.0617733770510003No Hit
CGCTTCAAGTCTCAGCCCGAG1437550.8673244830096288No Hit
GACCGCGACCTCGGAAAGGG1198000.7227955414737125No Hit
GACCGCGACCTCGGAAAGGGA1182500.7134438462376169No Hit
GACCGCGACCTCGGAAAG1150950.6944086214183384No Hit
TGCTTCAAGTCTCAGCCCGAG972960.5870209933491347No Hit
GACCGCGACCTCGGAAAGGGAG965710.582646813319348No Hit
TGCGAGAATTCTACCACTGAACCAC933580.5632616540976866No Hit
TGACAGGGTCGAGTTCGACTATCTT882760.5326001604268235No Hit
GGCCAGTCCGGGCCGGATCC882010.5321476590444317No Hit
AACCGCTTCAAGTCTCAGCCCGAG836380.5046174749397192No Hit
GGCCAGTCCGGGCCGGATCCAAC790710.4770631574279459No Hit
AAACTAACCAGCCTAGACTTGA720920.43495639545845477No Hit
TCCACTTCAGAGCAAAGATTTTCA704550.4250797986187848No Hit
ATCCACTTCAGAGCAAAGATTTTCA601270.36276734158756185No Hit
ACAAGTTCGGATCTACGGGT577410.34837176427573985No Hit
GACCGCGACCTCGGAAAGG549720.33166541323783744No Hit
AACCGCTTCAAGTCTCAGCCCGAGA538430.32485375909490066No Hit
ACTCGTTCTCAAAGTAGTTCAGCAG529230.3193030754708956No Hit
GCCAGTCCGGGCCGGATCC524860.31666650074949315No Hit
CGCTTCAAGTCTCAGCCCGAGA500740.3021140562917753No Hit
GTAGACACTCAGACAGACATGCTCC485580.2929674950156973No Hit
AGCGAGAATTCTACCACTGAACCAC466150.28124469253586903No Hit
TGCCAGTCCGGGCCGGATCC434290.2620224338118686No Hit
CCACAAGTTCGGATCTACGGGT431250.26018829487524087No Hit
TGCCAGTCCGGGCCGGATCCAAC406360.24517128233160088No Hit
AGAATTCTACCACTGAACCACAGTG394540.2380398605451073No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG383580.2314273070104229No Hit
CTCCACTTCAGAGCAAAGATTTTCA370190.22334864899678933No Hit
ACAAATCTTAGAACAAAGGCTTAAT348620.2103347092392034No Hit
CCACTTCAGAGCAAAGATTTTCA340940.20570109508351214No Hit
TGCTTCAAGTCTCAGCCCGAGA338460.20420482384573682No Hit
ACTCGGTCTCGGCGAGGAAGAACAG329390.1987325737946796No Hit
GAGAATTCTACCACTGAACCACAAG328030.1979120379546093No Hit
CGGAAACGTTCGAGTTGGCTATCGG322380.1945031942072583No Hit
GGAATTCTACCACTGAACCACCAGT312490.1885362093114528No Hit
AGGCGAGAATTCTACCACTGAACCA308260.18598410151476347No Hit
AGCCGGGCTGTAACAGTCCGGAGCC297930.1797516491412881No Hit
TTCCCGGTCCGACCGCGACCTCGGA293530.17709697436459002No Hit
GGCCAATCCACGGCCGATTCCTTCC281380.1697664519698441No Hit
GCGAGAATTCTACCACTGAACCACC273660.16510870440709197No Hit
TCCCACTTCAGAGCAAAGATTTTCA270160.16299703128926393No Hit
GACAGGGTCGAGTTCGACTATCTTG270060.1629366977716117No Hit
TTCCACCTCAGAGCAAAGATTTTCA262950.15864698466653815No Hit
GCTTCAAGTCTCAGCCCGAG250610.151201828588253No Hit
TCATGCTCTACCGACTGAGCTAGCC250470.15111736166353987No Hit
ACAGCTGGGCCAATCCACGGCCGAT249980.15082172742704394No Hit
GCCTCGACCACGGCCAGTCCG228740.13800688827771035No Hit
TACCGCGACCTCGGAAAGGGA227080.13700535188468332No Hit
TACCGCGACCTCGGAAAGGG224750.1355995809233864No Hit
TACCGCGACCTCGGAAAG223680.1349540122845075No Hit
TGGAAACGTTCGAGTTGGCTATCGG214980.12970499624876353No Hit
GGGCCGGATCCAACCGCTTCAAGTC211100.127364055763857No Hit
TTCCACTTCAGAGCAAAGATTTTCG202860.1223925739093133No Hit
AGCCAGTCCGGGCCGGATCC202060.12190990576809546No Hit
AGTCCGGGCCGGATCCAAC198540.11978616594673698No Hit
TACCGCGACCTCGGAAAGGGAG190750.11508618492162828No Hit
GCCAGAATTGCCTGAAGCCGTGCAG186820.11271507767789564No Hit
GGCCAGTCCGGGCCGGATCCA186670.11262457740141729No Hit
GTCGAGTTCGACTATCTTGGTC184040.11103780588716365No Hit
TGAATTCTACCACTGAACCACCAGT183390.11064563802242416No Hit
TAGAATTCTACCACTGAACCACCAG180570.10894423282463128No Hit
CCAGAATTGCCTGAAGCCGTGCAGG180050.10863049853283968No Hit
AGCACACTCGGTCTCGGCGAGGAAG177340.10699546020446425No Hit
AGCCAGTCCGGGCCGGATCCAAC174130.10505875428782768No Hit
TGGCGAGAATTCTACCACTGAACCA172160.10387018399007876No Hit
ACACTCGGTCTCGGCGAGGAAGAAC166180.10026223963447542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)